2011 Fiscal Year Final Research Report
Construction of human antibody library and establishment of selection method to develop fragment antibody therapeutics
| Project/Area Number |
21390032
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Drug development chemistry
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
ITO Yuji 鹿児島大学, 大学院・理工学研究科, 教授 (60223195)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ARIMA Naomichi 鹿児島大学, 大学院・医歯学総合研究科, 教授 (30175997)
KUROKI Ryota 独立行政法人日本原子力研究開発機構, 量子ビーム応用研究部門, ユニット長 (30391246)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 医薬分子機能学 |
|---|
| Research Abstract |
In this study, we constructed the libraries of human single-chain Fv antibody and llama VHH antibody using T7 phage display system and established the selection method to isolate the specific phages. Especially, we succeeded in the isolation of the T7 phage clones binding to the antigens from the library, which indicates our library system is useful for a rapid generation of specific antibodies. On the other hand, we found the problematic issues that the non-specific binding phages were enriched by selection process because of folding problems and instability of antibody, which should be solved in the further investigations.
|
-
[Journal Article] A mimotope peptide of Aβ42 fibril-specific antibodies with Aβ42 fibrillation inhibitory activity induces anti-Abeta42 conformer antibody response by a displayed form on an M13 phage in mice2011
Author(s)
Tanaka, K., Nishimura, M., Yamaguchi, Y., Hashiguchi, S., Takiguchi, S., Yamaguchi, M., Tahara, H., Gotanda, T., Abe, R., Ito, Y., Sugimura, K.
-
Journal Title
Journal of neuroimmunology
Volume: 236
Pages: 27-38
DOI
Peer Reviewed
-
[Journal Article] Characterization of human single-chain antibodies against highly pathogenic avian influenza H5N1 viruses : mimotope and neutralizing activity2010
Author(s)
Yang, J., Yoshida, R., Kariya, Y., Zhang, X., Hashiguchi, S., Nakashima, T., Suda, Y., Takada, A., Ito, Y., Sugimura, K.
-
Journal Title
Journal of biochemistry
Volume: 148
Pages: 507-515
DOI
Peer Reviewed
-
[Journal Article] Short communication : generation of recombinant monoclonal antibodies against an immunodominant HLA-A 2402-restricted HIV type 1 CTL epitope2009
Author(s)
Nunoya, J., Nakashima, T., Kawana-Tachikawa, A., Kiyotani, K., Ito, Y., Sugimura, K., Iwamoto, A.
-
Journal Title
AIDS research and human retroviruses
Volume: 25
Pages: 897-904
DOI
Peer Reviewed
-
[Journal Article] Effective induction of cell death on adult T-cell leukaemia cells by HLA-DRbeta-specific small antibody fragment isolated from human antibody phage library2009
Author(s)
Muraoka, S., Ito, Y., Kamimura, M., Baba, M., Arima, N., Suda, Y., Hashiguchi, S., Torikai, M., Nakashima, T., Sugimura, K.
-
Journal Title
Journal of biochemistry
Volume: 145
Pages: 799-810
DOI
Peer Reviewed
-
[Journal Article] Regulation of T cell response by blocking the ICOS signal with the B7RP-1-specific small antibody fragment isolated from human antibody phage library2009
Author(s)
Maeda, M., Ito, Y., Hatanaka, T., Hashiguchi, S., Torikai, M., Nakashima, T., Sugimura, K.
-
Journal Title
MAbs
Volume: 1
Pages: 453-461
URL
Peer Reviewed
-
-
-
-
-
-
-
-
-
