2013 Fiscal Year Final Research Report
The development of new technology to reanimation for endangered species.
| Project/Area Number |
21688020
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | University of Yamanashi (2013)
The Institute of Physical and Chemical Research (2009-2012) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2009-04-01 – 2014-03-31
|
| Keywords | クローン / 絶滅動物 / 染色体移植 |
|---|
| Research Abstract |
In this study, I tried to develop new technology to reanimate for extinct or endangered species. First, I had improved the basic nuclear transfer techniques, such as collection of intact donor nuclei from dead body, period of oocyte activation, and HDACi treatment. Second, mouse dried skin, preserved at room temperature for more than 1 year were used as the model of real animal. Although cloned blastocysts were obtained from preserved skin nucleus, those embryos failed to develop beyond blastocyst after embryo transfer. Third, I tried to repeat the nuclear transfer from somatic cell of cloned mouse to examine the possibility of infinite cloning. As the results, so far I could generate over 700 cloned mice from only one donor mouse by 30 times repeating of nuclear transfer.
|
-
[Journal Article] Successful serial recloning in the mouse over multiple generations2013
Author(s)
Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T
-
Journal Title
Cell Stem Cell
Volume: 7, (カバー採用)
Pages: 293-297
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
