2010 Fiscal Year Final Research Report

Regulation of translation machinery and mRNA localization by PAR-aPKC complex

Research Project

Project/Area Number	21770190
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Molecular biology
Research Institution	Yokohama City University
Principal Investigator	HAYASHI Kenji Yokohama City University -> 横浜市立大学, 医学研究科, 博士研究員 (50512349)
Project Period (FY)	2009 – 2010
Keywords	PAR1b / MARK2 / Microtubule / Dendritic spine / +TIPs
Research Abstract	We revealed that PAR1b participates in the maintenance of mature dendritic spine morphology in mouse hippocampal neurons. PAR1b localized in the dendrites of mature neurons, and PAR1b knockdown cells exhibited decreased mushroom-like dendritic spines, as well as increased filopodia-like dendritic protrusions. We revealed that microtubule growth distance in dendrite region is decreased following PAR1b knockdown. In addition, reduced accumulation of GFP-p140Cap, an actin reorganizing protein, in dendritic protrusions was confirmed in PAR1b knockdown neurons. In conclusion, the present results suggested a novel function for PAR1b in the maintenance of mature dendritic spine morphology by regulating microtubule growth and the accumulation of p140Cap in dendritic spines.

(2 results)