2010 Fiscal Year Final Research Report
Regulation of translation machinery and mRNA localization by PAR-aPKC complex
| Project/Area Number |
21770190
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Yokohama City University |
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Principal Investigator |
HAYASHI Kenji Yokohama City University, 医学研究科, 博士研究員 (50512349)
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| Project Period (FY) |
2009 – 2010
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| Keywords | PAR1b / MARK2 / Microtubule / Dendritic spine / +TIPs |
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| Research Abstract |
We revealed that PAR1b participates in the maintenance of mature dendritic spine morphology in mouse hippocampal neurons. PAR1b localized in the dendrites of mature neurons, and PAR1b knockdown cells exhibited decreased mushroom-like dendritic spines, as well as increased filopodia-like dendritic protrusions. We revealed that microtubule growth distance in dendrite region is decreased following PAR1b knockdown. In addition, reduced accumulation of GFP-p140Cap, an actin reorganizing protein, in dendritic protrusions was confirmed in PAR1b knockdown neurons. In conclusion, the present results suggested a novel function for PAR1b in the maintenance of mature dendritic spine morphology by regulating microtubule growth and the accumulation of p140Cap in dendritic spines.
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