Development of novel efficient techniques for genetic manipulation and their application to explore the mechanisms of body axis determination in the cricket Gryllus bimaculatus

2010 Fiscal Year Final Research Report

• Project/Area Number: 21770237
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: The University of Tokushima
• Principal Investigator: MITO Taro The University of Tokushima, 大学院・ソシオテクノサイエンス研究部, 助教 (80322254)
• Project Period (FY): 2009 – 2010
• Keywords: 遺伝子導入 / 昆虫 / 体軸決定

Research Abstract

We established techniques of genetic modification (transgenesis and targeted mutagenesis) in the cricket Gryllus bimaculatus, of which the modes of oogenesis and embryogenesis differ from those of Drosophila. Using the transgenic cricket system, we investigated cell behaviors during early embryogenesis via live imaging of fluorescently labeled embryonic cells/nuclei and revealed very dynamic features of blastoderm cells. Furthermore, results from our live imaging analysis of RNAi-treated embryos suggested that the presumptive embryonic cells are regionally specified by genes such as orthodenticle before the dynamic cell movement in late blastoderm stages.

Research Products

• [Journal Article] Regulation of leg size and shape : involvement of the Dachsous/Fat signaling pathway. (2011)
  • Author(s): Bando T, Mito T, Nakamura T, Ohuchi H, Noji S.
  • Journal Title: Developmental Dynamics in press
  • Peer Reviewed
• [Journal Article] The advent of RNA interference in Entomology. (2011)
  • Author(s): Mito T, Nakamura T, Band T, Ohuchi H, Noji S
  • Journal Title: Entomological Science 14 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism. (2010)
  • Author(s): Nakamura T, Yoshizaki M, Ogawa S, Okamoto H, Shinmyo Y, Bando T, Ohuchi H, Noji S, Mito T.
  • Journal Title: Curr Biol. 20(18) Pages: 1641-1647
  • Peer Reviewed
• [Journal Article] Evolution of insect development : to the hemimetabolous paradigm. (2010)
  • Author(s): Mito T, Nakamura T, Noji S.
  • Journal Title: Curr Opin Genet Dev. 20(4) Pages: 355-361
  • Peer Reviewed
• [Presentation] Functions of the orthodenticle-related genes during embryogenesis in the cricket Gryllus bimaculatus. (2010)
  • Author(s): 吉崎正人, 他
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 20101207-20101210
• [Presentation] Highly dynamic cell behavior during early development in the intermediate germ insect Gryllus bimaculatus, as revealed by analyses of transgenic embryos. (2010)
  • Author(s): Mito T, et al.
  • Organizer: 51th Annual Drosophila Research Conference.
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20100407-20100411
• [Presentation] Dynamic control of positional specification in a primitive mode of insect segmentation. (2010)
  • Author(s): Nakamura T, et al.
  • Organizer: 3rd EuroEvoDevo Conference.
  • Place of Presentation: パリ第7大学(フランス)
  • Year and Date: 2010-07-08
• [Presentation] 祖先的な昆虫における体節形成のダイナミックな制御 (2010)
  • Author(s): 中村太郎, 他
  • Organizer: 日本発生生物学会第43回大会(APDBN共催)
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-06-22
• [Presentation] 新規モデル生物:発生と再生研究に有用なコオロギ(Gryllus bimaculatus) (2009)
  • Author(s): 三戸太郎
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] RNAi法を用いた昆虫(コオロギ)の発生・再生メカニズムの研究 (2009)
  • Author(s): 三戸太郎
  • Organizer: 日本比較生理生化学会第31回大会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-22
• [Presentation] Dynamics of cellular aggregation and differentiation during early development of transgenic crickets. (2009)
  • Author(s): Nakamura, T, 他
  • Organizer: 16th International Society of Developmental Biologists Congress.
  • Place of Presentation: Edinburgh,UK
  • Year and Date: 2009-09-07
• [Presentation] Cellular dynamics during blastoderm stages and early embryogenesis, as revealed by transgenic cricket, Gryllus bimaculatus. (2009)
  • Author(s): Nakamura, T, 他
  • Organizer: 日本発生生物学会第42回大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-30
• [Book] 古生物学事典 第2版(棚部一成,北里洋編)(発生と進化に関連する9項目を担当) (2010)
  • Author(s): 三戸太郎
  • Publisher: 朝倉書店(著書,分担執筆)
• [Patent(Industrial Property Rights)] トランスジェニック不完全変態類昆虫の作成方法、トランスジェニック不完全変態類昆虫の卵の作成方法、トランスジェニック不完全変態昆虫およびキット (2009)
  • Inventor(s): 野地澄晴, 大内淑代, 三戸太郎, 中村太郎, 三ツ森正之
  • Industrial Property Rights Holder: 国立大学法人徳島大学,アロカ株式会社/野地澄晴, 大内淑代, 三戸太郎, 中村太郎, 三ツ森正之
  • Industrial Property Number: 特許,特願2009-238841
  • Filing Date: 2009-10-16