2010 Fiscal Year Final Research Report
Fermentative production of plant alkaloids in L-tyrosine producing Escherichia coli.
| Project/Area Number |
21780080
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
MINAMI Hiromichi Ishikawa Prefectural University, 生物資源環境学部, 助教 (90433200)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 植物アルカロイド / レチクリン / 発酵生産 |
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| Research Abstract |
We generated an E. coli strain that over-produces L-tyrosine by two steps of genetic engineering. An E. coli strain has disrupted the tyrR gene, the product of which represses the expression of genes involved in aromatic amino acid biosynthesis. In addition, the feedback-inhibition-resistant (fbr) 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, fbr-chorismate mutase/prephenate dehydrogenase, phosphoenolpyruvate synthetase, and transketolase are over-expressed in an E. coli strain disrupted the tyrR gene. We establish an E. coli fermentation system that yields plant alkaloids from simple carbon sources, introducing tyrosinase from Ralstonia solanacearum, DOPA decarboxylase from Pseudomonas putida, and reticuline biosynthetic enzymes from dopamine in the L-tyrosine over-producing E. coli strain. The fermentation platform described here offers opportunities for low-cost production of many diverse alkaloids.
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