2010 Fiscal Year Final Research Report
Role of arginine-methyltransferase for quality control of protein maturation
Project/Area Number |
21790181
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Osaka University |
Principal Investigator |
MATSUZAKI Shinsuke Osaka University, 連合小児発達学研究科, 准教授 (60403193)
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Project Period (FY) |
2009 – 2010
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Keywords | 小胞体ストレス / アルギニンメチル化酵素 / タンパク質品質管理 / ジメチルアルギニン / PRMT1 |
Research Abstract |
We discovered upregulation of Protein Arginine Methyltransferases (PRMT) under endoplasmic reticulum (ER) stress condition. Protein level of PRMT1 is increased by ER stressor tunicamycin, time-dependently. PRMT1 is upregulated by XBP1 overexpression. In contrast, cells knocked-down (KD) of PRMT1 showed increase of ER-chaperone GRP78 under untreated condition (non-stress condition). Moreover, these cells cause abnormal localization Golgi complex, resulting increase of the Golgi-related proteins such as GM130, TGN38, Fut8. Finally, to transfect PRMT1-YFP into PRMT1-KD cells, the abnormal accumulation of Golgi complex was recovered.These results suggest that PRMT1 regulates the structure and the function of Golgi complex, associated to ER function.
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[Journal Article] DISC1 regulates cell-cell adhesion, cell-matrix adhesion and neurite outgrowth.2010
Author(s)
Hattori T, Shimizu S, Koyama Y, Yamada K, Kuwahara R, Kumamoto N, Matsuzaki S, Ito A, Katayama T, Tohyama M.
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Journal Title
Molecular Psychiatry 15(8)
Pages: 778, 798-809
Peer Reviewed
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[Journal Article] Dysbindin regulates the transcriptional level of myristoylated alanine-rich protein kinase C substrate via the interaction with NF-YB in mice brain.2010
Author(s)
Okuda H, Kuwahara R, Matsuzaki S, Miyata S, Kumamoto N, Hattori T, Shimizu S, Yamada K, Kawamoto K, Hashimoto R, Takeda M, Katayama T, Tohyama M.
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Journal Title
PLoS ONE.(corresponding author and equally contributed author) 5(1)
Pages: e8773
Peer Reviewed
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[Journal Article] Increased stathminl expression in the dentate gyrus of mice causes abnormal axonal arborizations.2010
Author(s)
Yamada K, Matsuzaki S, Hattori T, Kuwahara R, Taniguchi M, Hashimoto H, Shintani N, Baba A, Kumamoto N, Yamada K, Yoshikawa T, Katayama T, Tohyama M.
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Journal Title
PLoS ONE. 5(1)
Pages: e8596
Peer Reviewed
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[Journal Article] Association studies and gene expression analyses of the DISCl-interacting molecules, pericentrin 2 (PCNT2) and DISCl-binding zinc finger protein (DBZ), with schizophrenia and with bipolar disorder.2009
Author(s)
Anitha A, Nakamura K, Yamada K, Iwayama Y, Toyota T, Takei N, Iwata Y, Suzuki K, Sekine Y, Matsuzaki H, Kawai M, Thanseem I, Miyoshi K, Katayama T, Matsuzaki S, Baba K, Honda A, Hattori T, Shimizu S, Kumamoto N, Kikuchi M, Tohyama M, Yoshikawa T, Mori N.
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Journal Title
Am J Med Genet B Neuropsychiatr Genet. 150B(7)
Pages: 967-76
Peer Reviewed
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[Presentation] ERストレス下での翻訳後修飾2009
Author(s)
桑原隆亮, 松崎伸介, 服部剛志, 森泰丈, 河本恵介, 清水尚子, 山田浩平, 片山泰一, 遠山正彌
Organizer
第32回神経科学大会
Place of Presentation
名古屋市
Year and Date
20090916-20090918
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