Role of arginine-methyltransferase for quality control of protein maturation

2010 Fiscal Year Final Research Report

• Project/Area Number: 21790181
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka University
• Principal Investigator: MATSUZAKI Shinsuke Osaka University, 連合小児発達学研究科, 准教授 (60403193)
• Project Period (FY): 2009 – 2010
• Keywords: 小胞体ストレス / アルギニンメチル化酵素 / タンパク質品質管理 / ジメチルアルギニン / PRMT1

Research Abstract

We discovered upregulation of Protein Arginine Methyltransferases (PRMT) under endoplasmic reticulum (ER) stress condition. Protein level of PRMT1 is increased by ER stressor tunicamycin, time-dependently. PRMT1 is upregulated by XBP1 overexpression. In contrast, cells knocked-down (KD) of PRMT1 showed increase of ER-chaperone GRP78 under untreated condition (non-stress condition). Moreover, these cells cause abnormal localization Golgi complex, resulting increase of the Golgi-related proteins such as GM130, TGN38, Fut8. Finally, to transfect PRMT1-YFP into PRMT1-KD cells, the abnormal accumulation of Golgi complex was recovered.These results suggest that PRMT1 regulates the structure and the function of Golgi complex, associated to ER function.

Research Products

• [Journal Article] Reduced rate of neural differentiation in the dentate gyrus of adult dysbindin null (Sandy) mouse. (2011)
  • Author(s): Nihonmatsu-Kikuchi N, Hashimoto R, Hattori S, Matsuzaki S, Shinozaki T, Miura H, Ohota S, Tohyama M, Takeda M, Takebayashi Y
  • Journal Title: PLoS ONE. 6(1) Pages: e15886
  • Peer Reviewed
• [Journal Article] Familiar amyotrophic lateral sclerosis (FALS)-linked SOD1 mutation accelerates neuronal cell death by activating cleavage of caspase-4 under ER stress in an in vitro model of FALS. (2010)
  • Author(s): Koyama Y, Hiratsuka T, Matsuzaki S, Yamagishi S, Kato S, Katayama T, Tohyama M.
  • Journal Title: Neurochem Int.(equally contributed author) 57(7) Pages: 838-43
  • Peer Reviewed
• [Journal Article] Yokukansan inhibits neuronal death during ER stress by regulating the unfolded protein response. (2010)
  • Author(s): Hiratsuka T, Matsuzaki S, その他6名(8人中2番目)
  • Journal Title: PLoS ONE.(corresponding author and equally contributed author) 5(10) Pages: e13280
  • Peer Reviewed
• [Journal Article] bFGF regulates PI3-kinase-Rac1-JNK pathway and promotes fibroblast migration in wound healing. (2010)
  • Author(s): Kanazawa S, Fujiwara T, Matsuzaki S, Shingaki K, Taniguchi M, その他6名(11人中3番目).
  • Journal Title: PLoS ONE. 5(8) Pages: ii : e12228
  • Peer Reviewed
• [Journal Article] Molecular mechanism of kallikrein-related peptidase 8/neuropsin-induced hyperkeratosis in inflamed skin. (2010)
  • Author(s): Shingaki K, Matsuzaki S, Taniguchi M, Kubo T, その他9名(13人中2番目)
  • Journal Title: Br J Dermatol.(equally contributed author) 163(3) Pages: 466-75
  • Peer Reviewed
• [Journal Article] DISC1 regulates cell-cell adhesion, cell-matrix adhesion and neurite outgrowth. (2010)
  • Author(s): Hattori T, Shimizu S, Koyama Y, Yamada K, Kuwahara R, Kumamoto N, Matsuzaki S, Ito A, Katayama T, Tohyama M.
  • Journal Title: Molecular Psychiatry 15(8) Pages: 778, 798-809
  • Peer Reviewed
• [Journal Article] Dysbindin regulates the transcriptional level of myristoylated alanine-rich protein kinase C substrate via the interaction with NF-YB in mice brain. (2010)
  • Author(s): Okuda H, Kuwahara R, Matsuzaki S, Miyata S, Kumamoto N, Hattori T, Shimizu S, Yamada K, Kawamoto K, Hashimoto R, Takeda M, Katayama T, Tohyama M.
  • Journal Title: PLoS ONE.(corresponding author and equally contributed author) 5(1) Pages: e8773
  • Peer Reviewed
• [Journal Article] Increased stathminl expression in the dentate gyrus of mice causes abnormal axonal arborizations. (2010)
  • Author(s): Yamada K, Matsuzaki S, Hattori T, Kuwahara R, Taniguchi M, Hashimoto H, Shintani N, Baba A, Kumamoto N, Yamada K, Yoshikawa T, Katayama T, Tohyama M.
  • Journal Title: PLoS ONE. 5(1) Pages: e8596
  • Peer Reviewed
• [Journal Article] Caspase-4 is partially cleaved by calpain via the impairment of Ca2+ homeostasis under the ER stress. (2010)
  • Author(s): Matsuzaki S, Hiratsuka T, Kuwahara R, Katayama T, Tohyama M.
  • Journal Title: Neurochem Int.(First author and corresponding author) 56(2) Pages: 352-6
  • Peer Reviewed
• [Journal Article] Role of the PACAP-PAC1-DISC1 and PACAP-PAC1-stathminl systems in schizophrenia and bipolar disorder : novel treatment mechanisms? (2009)
  • Author(s): Katayama T, Hattori T, Yamada K, Matsuzaki S, Tohyama M.
  • Journal Title: Pharmacogenomics. 1967-78 Pages: 1967-78
  • Peer Reviewed
• [Journal Article] Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice. (2009)
  • Author(s): Miyoshi K, Kasahara K, Miyazaki I, Shimizu S, Taniguchi M, Matsuzaki S, Tohyama M, Asanuma M.
  • Journal Title: FASEB J. 23(10) Pages: 3289-97
  • Peer Reviewed
• [Journal Article] Association studies and gene expression analyses of the DISCl-interacting molecules, pericentrin 2 (PCNT2) and DISCl-binding zinc finger protein (DBZ), with schizophrenia and with bipolar disorder. (2009)
  • Author(s): Anitha A, Nakamura K, Yamada K, Iwayama Y, Toyota T, Takei N, Iwata Y, Suzuki K, Sekine Y, Matsuzaki H, Kawai M, Thanseem I, Miyoshi K, Katayama T, Matsuzaki S, Baba K, Honda A, Hattori T, Shimizu S, Kumamoto N, Kikuchi M, Tohyama M, Yoshikawa T, Mori N.
  • Journal Title: Am J Med Genet B Neuropsychiatr Genet. 150B(7) Pages: 967-76
  • Peer Reviewed
• [Presentation] SUMO1 Conjugates Indentified in an Over-expressing Transgenic Mouse Model and their Links to Disease and Synaptic Function (2011)
  • Author(s): Shinsuke Matsuzaki, Taiichi Katayama, Masaya Tohyama, Brian Raught, Paul Fraser
  • Organizer: The 10th International Conference on Alzheimer's and Parkinson's Diseases.
  • Place of Presentation: バルセロナ
  • Year and Date: 20110309-20110313
• [Presentation] Brian Raught and Paul Fraser., Neuronal Sumoylation and Its Contributions to Form, Function and Disease、EMBO Workshop, Proteolysis & Neurodegeneration (2010)
  • Author(s): Shinsuke Matsuzaki, Taiichi Katayama, Masaya Tohyama, Brian Raught, Paul Fraser.
  • Organizer: 5th Inproteolys Meeting
  • Place of Presentation: マドリッド
  • Year and Date: 20100504-20100507
• [Presentation] DISC1 regulates cell-cell adhesion, cell-matrix adhesion and neurite outgrowth. (2010)
  • Author(s): Hattori T, Shimizu S, Yamada K, Kuwahara R, Matsuzaki S, et al.
  • Organizer: International Symposium "DISC1 2010" (Edinburgh)
  • Place of Presentation: Edinburgh First John McIntyre Conference Centre, Edinburgh, Scotland(UK)
  • Year and Date: 2010-09-04
• [Presentation] Analysis of Protein Arginine Methyltrasferase under ER stress. (2010)
  • Author(s): 桑原隆亮、松崎伸介、他.
  • Organizer: 第53回日本神経化学会大会(Neuro2010)
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2010-09-04
• [Presentation] 海馬歯状回でのstathmin1の増加は異常な軸索伸展を惹起する (2010)
  • Author(s): 山田浩平、松崎伸介, 他
  • Organizer: 第53回日本神経化学会大会(Neuro2010)
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2010-09-03
• [Presentation] Inhibitory effect of Ferulic acid against ER stress could suggest the clinical validity of Yokukansan, a Japanese herbal medicine. (2010)
  • Author(s): 平塚徹、松崎伸介, 他
  • Organizer: 第53回日本神経化学会大会(Neuro2010)
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2010-09-02
• [Presentation] 抑肝散に含まれるフェルラ酸はERストレスによる神経細胞死を抑制する (2010)
  • Author(s): 平塚徹、松崎伸介, 他.
  • Organizer: 第27回和漢医薬学会学術大会
  • Place of Presentation: 京都薬科大学(京都市)
  • Year and Date: 2010-08-29
• [Presentation] ERストレス下での翻訳後修飾 (2009)
  • Author(s): 桑原隆亮, 松崎伸介, 服部剛志, 森泰丈, 河本恵介, 清水尚子, 山田浩平, 片山泰一, 遠山正彌
  • Organizer: 第32回神経科学大会
  • Place of Presentation: 名古屋市
  • Year and Date: 20090916-20090918
• [Presentation] 小胞体ストレスを起源とするアルツハイマー氏病発症機構への抑肝散の効果 (2009)
  • Author(s): 平塚徹, 松崎伸介, 宮田信吾, 片山泰一, 遠山正彌
  • Organizer: 第32回神経科学大会
  • Place of Presentation: 名古屋市
  • Year and Date: 20090916-20090918
• [Presentation] DISC1の細胞接着性への影響の検討 (2009)
  • Author(s): 服部剛志, 清水尚子, 山田浩平, 桑原隆亮, 大野浩司, 松崎伸介, 片山泰一, 伊藤彰, 遠山正彌
  • Organizer: 第32回神経科学大会
  • Place of Presentation: 名古屋市
  • Year and Date: 20090916-20090918
• [Presentation] 統合失調症関連因子PACAPによる神経分化調節機構の解析 (2009)
  • Author(s): 山田浩平、松崎伸介、服部剛志、橋本均、新谷紀人、馬場明道、片山泰一、遠山正彌
  • Organizer: 第52回日本神経化学会シンポジウム
  • Place of Presentation: 渋川市
  • Year and Date: 20090622-20090624
• [Presentation] 統合失調症脆弱性遺伝子ディスバインジンと記憶との関連:ヒトと動物モデルの表現型の検討 (2009)
  • Author(s): 橋本亮太、高雄啓三、服部聡子、室谷知孝、遠山桂子、中西和男、松崎伸介、石塚智子、熊本奈都子、高村明孝、大井一高、福本素由己、山森英長、安田由華、遠山正彌、大和谷厚、功刀浩、宮川剛、武田雅俊
  • Organizer: 第31回日本生物学的精神医学会
  • Place of Presentation: 京都市
  • Year and Date: 20090423-20090425