2010 Fiscal Year Final Research Report
Morphological study on the cell membrane kinetics in gastric parietal cells associated with gastric acid secretion.
| Project/Area Number |
21790185
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Miyazaki |
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Principal Investigator |
SAWAGUCHI Akira University of Miyazaki, 医学部, 教授 (30336292)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 高圧凍結技法 / 胃底腺 / 超微形態 / 壁細胞 / プロトンポンプ / 単離胃粘膜 / 回復期 |
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| Research Abstract |
To elucidate a functional transformation of gastric parietal cells, we have newly developed an isolated rat gastric mucosa model whose parietal cells exhibited a reverting process from the active to resting state of acid secretion. Immunohistochemistry of H^+/K^+-ATPase demonstrated a progressive translocation of H^+/K^+-ATPase from the apical to the cytoplasmic region. In the reverting process, the apical membranes were internalized into the cytoplasm forming unique penta-laminar membranes which were labeled for autophagosome-marker LC3. This finding suggested an association of autophagy with the reverting process in the gastric parietal cell.
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