2010 Fiscal Year Final Research Report
Ribosome abnormalities and cancer : Understanding the role of ribosomal proteins (RPs) in tumorigenesis and p53 regulation
| Project/Area Number |
21790288
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | University of Miyazaki |
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Principal Investigator |
CHAKRABORTY Anirban University of Miyazaki, フロンティア科学実験総合センター, 研究員 (40525565)
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| Project Period (FY) |
2009 – 2010
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| Keywords | リボソームタンパク質 / アポトーシス / がん / ゼブラフィッシュ |
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| Research Abstract |
It is now widely accepted that disruption of ribosome biogenesis triggers a p53 pathway and studies in cell lines have shown that several RPs are crucial players in this ribosome-p53 signaling pathway. Interestingly, human ribosomal diseases caused due to mutations in RP genes often present with increased cancer susceptibility. However, the molecular mechanism that links RPs to p53 regulation remains unknown. To investigate the role of RPs in p53 regulation and cancer, we undertook the development of tissue-specific RP knockdown model in zebrafish, which is a powerful cancer model system. We have found that tissue specific knockdown of RP gene using miRNA can be effectively carried out using the Tol2 transposase system. This technique will be a valuable tool to develop and understand cancer in zebrafish.
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