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Ribosome abnormalities and cancer : Understanding the role of ribosomal proteins (RPs) in tumorigenesis and p53 regulation

Research Project

Project/Area Number 21790288
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionUniversity of Miyazaki

Principal Investigator

CHAKRABORTY Anirban  University of Miyazaki, フロンティア科学実験総合センター, 研究員 (40525565)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsリボソームタンパク質 / アポトーシス / がん / ゼブラフィッシュ / 翻訳異常 / p53
Research Abstract

It is now widely accepted that disruption of ribosome biogenesis triggers a p53 pathway and studies in cell lines have shown that several RPs are crucial players in this ribosome-p53 signaling pathway. Interestingly, human ribosomal diseases caused due to mutations in RP genes often present with increased cancer susceptibility. However, the molecular mechanism that links RPs to p53 regulation remains unknown. To investigate the role of RPs in p53 regulation and cancer, we undertook the development of tissue-specific RP knockdown model in zebrafish, which is a powerful cancer model system. We have found that tissue specific knockdown of RP gene using miRNA can be effectively carried out using the Tol2 transposase system. This technique will be a valuable tool to develop and understand cancer in zebrafish.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (11 results)

All 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results)

  • [Journal Article] Erythropoiesis failure due to RPS19deficiency is independent of an activated Tp53 response in a zebrafish model of Diamond-Blackfan anaemia.2011

    • Author(s)
      Torihara H, Uechi T, Chakraborty A, Shinya M, Sakai N, Kenmochi N.
    • Journal Title

      The British Journal of Haematology. 152

      Pages: 3342-3350

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Erythropoiesis failure due to RPS19 deficiency is independent of an activated Tp53 response in a zebrafish model of Diamond-Blackfan anaemia.2011

    • Author(s)
      Hidetsugu Torihara
    • Journal Title

      The British Journal of Haematology

      Volume: 152 Pages: 3342-3350

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Guarding the'translation apparatus' : defective ribosome biogenesis and the p53 signaling pathway.

    • Author(s)
      Chakraborty A, Uechi T, Kenmochi N.
    • Journal Title

      WIREs RNA. in press.

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Guarding the 'translation apparatus' : defective ribosome biogenesis and the p53 signaling pathway.

    • Author(s)
      Anirban Chakraborty
    • Journal Title

      WIREs RNA

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Ribosomal Protein L11(RPL11)Deficient Zebrafish Exhibit Upregulation of p53-Modulatory Nucleolar Proteins Involved in Ribosome Biogenesis.2010

    • Author(s)
      Anirban Chakraborty
    • Organizer
      9th International Meeting on Zebrafish Development and Genetics
    • Place of Presentation
      マディソン、米国
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ribosome dysfunction and nucleolar stress : An activated p53 response correlates with aberrant expression of nucleolar proteins in ribosomal protein-deficient zebrafish.2010

    • Author(s)
      Anirban Chakraborty, Tamayo Uechi, Gnaneshwar Yadav, Naoya Kenmochi.
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Ribosomal Protein L11(RPL11)Deficient Zebrafish Exhibit Upregulation of p53-Modulatory Nucleolar Proteins Involved in Ribosome Biogenesis.2010

    • Author(s)
      Anirban Chakraborty, Tamayo Uechi, Hidetsugu Torihara, Naoya Kenmochi.
    • Organizer
      International Meeting on Zebrafish Development and Genetics.
    • Place of Presentation
      マディソン(米国)
    • Related Report
      2010 Final Research Report
  • [Presentation] Ribosomal stress and the p53 network : Impaired ribosome biogenesisdue to ribosomal protein (RP) deficiency leads to p53 induction in zebrafish2009

    • Author(s)
      Anirban Chakraborty
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Ribosomal Protein L11 (Rp111) Deficiency In Zebrafish Leads to a Selective Upregulation of p53-Modulatory Nucleolar Proteins2009

    • Author(s)
      Anirban Chakraborty
    • Organizer
      6^<th> European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      ローマ, イタリア
    • Year and Date
      2009-07-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Ribosomal stress and the p53 network : Impaired ribosome biogenesis due to ribosomal protein(RP)deficiency leads to p53 induction in zebrafish.2009

    • Author(s)
      Anirban Chakraborty, Tamayo Uechi, Hidetsugu Torihara, Naoya Kenmochi.
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Ribosomal Protein L11(Rpl11)Deficiency In Zebrafish Leads to a Selective Upregulation of p53-Modulatory Nucleolar Proteins.2009

    • Author(s)
      Anirban Chakraborty, Tamayo Uechi, Hidetsugu Torihara, Naoya Kenmochi.
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      ローマ(イタリア)
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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