2010 Fiscal Year Final Research Report

Analyses of functions of deregulated miRNAs in renal carcinoma

Research Project

Project/Area Number	21790389
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Experimental pathology
Research Institution	Oita University
Principal Investigator	NAKADA Chisato Oita University, 医学部, 助教 (60379625)
Project Period (FY)	2009 – 2010
Keywords	microRNA / 腎細胞癌
Research Abstract	It has recently been reported that microRNAs (miRNAs), non-protein-coding small RNAs comprising about 22 nucleotides, are aberrantly expressed in certain carcinomas and play oncogenic or tumor-suppressive roles in carcinoma cells. The aim of this project is to clarify the functions of the miRNAs aberrantly expressed in clear cell renal cell carcinoma (CCC), and to identify miRNAs associated with carcinogenesis and/or cancer progression. Three miRNAs are found deregulated in CCC, but the mechanism and biological consequences of their deregulation are poorly understood. MiR-200c and miR-141 are significantly downregulated in CCC. Ectopic expression of these miRNAs induced growth suppression of renal carcinoma cell lines. Ectopic expression of miR-210, which is upregulated in CCC, caused accumulation at G2/M phase of the cell cycle associated with multipolar spindle, suggesting that miR-210 overexpression may trigger an event that hinders normal cell division.