2010 Fiscal Year Final Research Report

Study of an anti-tumor activity of adiponectin in a mouse lung metastasis model

Research Project

Project/Area Number	21790759
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Respiratory organ internal medicine
Research Institution	Asahikawa Medical College
Principal Investigator	TOYOSHIMA Eri Asahikawa Medical College, 医学部, 助教 (20466501)
Project Period (FY)	2009 – 2010
Keywords	アディポネクチン / 肺癌
Research Abstract	We used a tumor-bearing mouse model to evaluate an anti-tumor activity of adiponectin in lung cancer cells. Our results showed that both human and mouse adiponectin inhibited growth of A549 tumors. Next, we evaluated the expression levels of adiponectin receptor, AdipoR1 and AdipoR2 in A549 cells using RT-PCR assay. The assay revealed that A549 cells expressed both AdipoR1 and AdipoR2. Then, in vitro study, A549 cells were treated with adiponectin to study whether adiponectin suppresses cell proliferation of A549 cells. Cell proliferation assay showed that adiponectin did not suppress cell proliferation of the cells. After that, we used a mouse bone-marrow transplantation model to detect the bone-marrow derived cells (BMDCs) in xenograft tumors. Bone marrow cells from GFP-transgenic mice were transplanted to the irradiated mice, and A549 cells were implanted to the mice. GFP-labeled BMDCs decreased in adiponectin-treated tumors compared with control tumors.