2010 Fiscal Year Final Research Report
Preparation of chitosan microspheres for the medical application
| Project/Area Number |
21800035
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Okayama University |
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Principal Investigator |
SHIROSAKI Yuki Okayama University, 大学院・自然科学研究科, 助教 (40533956)
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| Project Period (FY) |
2009 – 2010
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| Keywords | キトサン / ミクロ粒子 / ミクロカプセル / 薬物徐放 / 細胞適合性 / 有機-無機複合体 |
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| Research Abstract |
In this study, Porous particles and capsules were prepared to recover of the damaged human tissues. Chitosan and γ-glycidoxypropyltrymethoxysilane (GPTMS) were used as the starting materials. Porous particles were prepared by dropping precursor sol with CaCl_2 or without into liquid nitrogen. Obtained porous particles had about 2 mm in diameter and 10-30μm in pore size. HAp particles of about 1μm in diameter deposited on the pore surface by soaking thus obtained hybrid parties in 0.01 M Na2HPO4 solution at 80℃ for 7 days. Bovine serum albumin (BSA) adsorbed much on the chitosan-GPTMS particles. Capsules were prepared by dropping carboxymethylcellulose into chitosan-GPTMS sols. The releasing of bioactive molecules included into the capsules was controlled by the cross-linking degree. Moreover the capsules did not show the cytotoxicity.
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