2010 Fiscal Year Final Research Report
Development of fluorescent strain sensor and imaging system
| Project/Area Number |
21870004
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
IWAI Sosuke Hirosaki University, 教育学部, 講師 (40366547)
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| Project Period (FY) |
2009 – 2010
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| Keywords | GFP / ストレインセンサー / スペクトル線形分離法 |
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| Research Abstract |
To detect external force-induced conformational changes of proteins in living cells, I improved and evaluated the GFP-based fluorescent strain sensor. Also, toward the establishment of an imaging system suitable for the sensor, I theoretically estimated the maximum errors in the ratio values of ratiometric sensors, obtained by linear spectral unmixing, which is based on the least squares regression method. The estimation would provide an useful criterion to validate the results obtained by spectral imaging of ratiometric sensors.
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