2010 Fiscal Year Final Research Report

Evaluation of molecular mechanisms of tumor-specific protoporphyr in accumulation induced by 5-aminolevulinic acid

Research Project

Project/Area Number	21890084
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Biological pharmacy
Research Institution	Kanazawa University
Principal Investigator	NAKANISHI Takeo Kanazawa University, 薬学系, 准教授 (30541742)
Co-Investigator(Renkei-kenkyūsha)	TAMAI Ikumi 金沢大学, 薬学系, 教授 (20155237) SHIRASAKA Yoshiyuki 金沢大学, 薬学系, 助教 (60453833)
Project Period (FY)	2009 – 2010
Keywords	トランスポーター / アミノレブリン酸 / プロトポルフィリン / 化学療法 / フェロキラターゼ / 光力学療法 / 有機アニオン
Research Abstract	5-Aminolevulinic acid is one of the most potent photodynamic therapeutic agents, because it induces tumor cell-specific intracellular accumulation of a photosensitizer, protoporphyrin IX (PPIX). However, molecular mechanisms of such 5-ALA-induced PPIX accumulation remain unclear. In the current study, in order to establish a basis to predict efficacy of photodynamic therapy to treat cancer, molecular mechanisms of intracellular accumulation of PPIX induced by 5-ALA were studied in various human cancer cell lines exposed to 5-ALA. Results suggested that PPIX accumulation is likely determined by PPIX biosynthesis, ferrochelatase (FECH) activity and PPIX efflux.

(1 results)