2023 Fiscal Year Final Research Report

Elucidation of the role of periodontal pathogenic bacteria-derived vesicles in periodontal medicine and development of preventive agents for the worsening of periodontal disease

Research Project

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Project/Area Number	21K09896
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 57030:Conservative dentistry-related
Research Institution	The University of Tokushima
Principal Investigator	YUMOTO Hiromichi 徳島大学, 大学院医歯薬学研究部(歯学域), 教授 (60284303)
Co-Investigator(Kenkyū-buntansha)	木戸淳一徳島大学, 大学院医歯薬学研究部(歯学域), 准教授 (10195315) 稲垣裕司徳島大学, 病院, 講師 (50380019) 廣島佑香徳島大学, 大学院医歯薬学研究部(歯学域), 講師 (60545143) 尾崎和美徳島大学, 大学院医歯薬学研究部(歯学域), 教授 (90214121)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	歯周病 / 歯周病原細菌 / Outer Membrane Vesicle / 歯肉上皮細胞 / 炎症性サイトカイン / 歯槽骨吸収 / 破骨細胞 / 骨芽細胞
Outline of Final Research Achievements	Vesicles derived from the periodontal pathogens, Porphyromonas gingivalis and Fusobacterium nucleatum, induced the expression and production of IL-6 and IL-8 in human gingival epithelial cells, and STING-NF-kB was involved in this pathway. Furthermore, Pg-OMV promoted osteoclast differentiation and inhibited osteoblast mineralization in vitro, and promoted alveolar bone formation in vivo. Pg-OMV also suppressed the expression of E-cadherin, an adhesion molecule involved in the epithelial barrier function, in human gingival epithelial cells, suggesting the influence of gingipain contained in Pg.
Free Research Field	医歯薬学
Academic Significance and Societal Importance of the Research Achievements	近年、歯周病と様々な全身疾患との関連が示されており、この負の連鎖を止める為に、歯周病原細菌由来OMVの役割を理解し、さらに口腔細菌感染の全身への波及過程とそのメカニズムを解明する事は、全身の健康へ寄与・QOL向上・健康寿命延伸のための治療や予防法の確立に繋がる。歯周病原細菌由来OMVが炎症を惹起し、歯槽骨吸収を促進するメカニズムの一端を解明した本研究成果は、歯周病の全身への波及過程の解明と新規の治療・予防法の確立に繋がることから、学術的意義に加えて、社会的意義も大きいと考えられる。