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2023 Fiscal Year Final Research Report

Non-destructive measurement method that overcomes light scattering to determine the sex of chicken eggs in early incubation

Research Project

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Project/Area Number 21K18228
Research Category

Grant-in-Aid for Challenging Research (Pioneering)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 41:Agricultural economics and rural sociology, agricultural engineering, and related fields
Research InstitutionKyoto University

Principal Investigator

Kondo Naoshi  京都大学, 農学研究科, 教授 (20183353)

Co-Investigator(Kenkyū-buntansha) 小川 雄一  京都大学, 農学研究科, 准教授 (20373285)
鈴木 哲仁  三重大学, 生物資源学研究科, 准教授 (00723115)
白神 慧一郎  京都大学, 農学研究科, 助教 (80795021)
Project Period (FY) 2021-07-09 – 2024-03-31
Keywords鶏卵 / 胚成長 / 非破壊計測 / レーザー / 透過画像 / 血管
Outline of Final Research Achievements

This research aims to solve the major unsolved problem of killing 7 billion male egg-laying hens after hatching. For this purpose, we aimed to develop a sensing technology for biological phenomena in the hen's egg, which are closely related to the sex of the egg, not only by conventional transmission spectroscopy but also by advanced nondestructive measurement techniques utilizing image analysis and laser speckle technology. As a result of investigating the light transmissivity of eggshells and their relationship with hemoglobin, we showed that it is possible to visualize the egg embryo using transmission images at around 500 nm. The image analysis results showed that the area and fractal dimension of the embryo and blood vessels on the third day of egg hatching were significantly more prominent in males than in females. On the other hand, experiments showed that speckle images can also be used to measure embryonic growth through the eggshell.

Free Research Field

生物センシング工学

Academic Significance and Societal Importance of the Research Achievements

鶏卵の非破壊雌雄判定法は,昨今のアニマルウェルフェアの観点からも極めて重要であり,世界的にも求められている技術である。鶏卵中の胚形成が孵化後の雌雄と関係があることは示唆されていたが,その成長に伴う血液量に着目した分光学的研究に留まっていた。本研究のように,鶏卵の光透過性を考慮し,胚成長を直接評価することで雌雄との関係を明らかにした例は少ない。また卵殻には点班が存在しており,その存在が透過画像の精度を低減させていることを明確にした。さらに,これを解決する方法としてスペックルイメージングが有効であることを見出し,特許出願を行っており,社会的意義も大きい研究であると言える。

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Published: 2025-01-30  

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