2022 Fiscal Year Final Research Report
Development of nose to brain delivery method using extracellular vesicles.
| Project/Area Number |
21K19908
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2021-07-09 – 2023-03-31
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| Keywords | 細胞外小胞 / エクソソーム |
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| Outline of Final Research Achievements |
In this study, we investigated the development of an intranasal brain delivery method using extracellular vesicles (EVs). The sEVs and esEVs were selected as EV of interest. EVs labeled with Gaussia luciferase (gLuc) was intranasally administered to mice. It was revealed that after intranasal administration of sEV and esEV, the EVs hardly migrated to the lungs and stomach, but migrated to the brain with high efficiency. There was no difference in the brain distribution rate between sEV and esEV. sEV was selected as target EV and the distribution of sEV in brain was evaluated. It was found that sEV migrated to a wide area of the brain, and was mainly taken up by microglia. These results indicate that EV is useful for nose to brain delivery,
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| Free Research Field |
生物薬剤学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、細胞外小胞（EV）を利用した経鼻脳デリバリー方法の開発について検討を行った。脳は重要臓器であることからこれへの薬物の送達による種々の疾患の治療法が期待されるが、脳と血管の間には脳―血管関門と呼ばれる障壁があるために、薬物の送達は困難である。経鼻投与は、脳―血管関門を介することなく脳へ薬物輸送が可能と期待できる。そこで、細胞から放出される膜小胞である細胞外小胞（EV）を利用した経鼻投与による脳デリバリーについて検証した。その結果、EVは経鼻脳デリバリーに有用であることを見出した。以上の成果は、EVを利用した中枢疾患治療薬の開発に有用な知見である。
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