Structure, formation, and contraction of the contractile ring

2014 Fiscal Year Final Research Report

• Project/Area Number: 22247031
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Gakushuin University
• Principal Investigator: MABUCHI Issei 学習院大学, 理学部, 教授 (40012520)
• Co-Investigator(Renkei-kenkyūsha): USUKURA Jiro 名古屋大学, エコトピア科学研究所, 教授 (30143415) ; KOJIMA Shin-ichiro 学習院大学, 理学部, 助教 (30519210) ; KASHIWAZAKI Jun 学習院大学, 理学部, 助教 (70570654)
• Research Collaborator: KASHIWAZAKI Jun ; TAKAGI Tomoko ; BALASUBRAMANIAN Mohan K. ; INOUE Shinya
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 細胞分裂 / 細胞質分裂 / 収縮環 / アクチン / ミオシン

Outline of Final Research Achievements

In cell division, cytokinesis takes place by contraction of the contractile ring at the mid region of the cell. I isolated the cell ghost from dividing fission east cells, in which the contractile ring was attached to the cell membrane, and demonstrated for the first time the contraction of the contractile ring by addition of ATP. It was shown using this system that actin depolymerization was not necessary in the contraction. It was also suggested that myosin V is involved in movement of actin to the division site during contractile ring formation. The actin polymerizing protein uDia was shown to co-localize with actin in the contractile ring of dividing sea urchin eggs. Furthermore, it was shown that actin flows in the frog egg extract encapsulated in artificial lipid vesicles.

Free Research Field

分子細胞生物学