A study to solve the mystery of AIDS pandemic by expeditions into the most remote areas in the Congo basin.

2013 Fiscal Year Final Research Report

• Project/Area Number: 22256001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Virology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: IDO Eiji 東京医科歯科大学, 医歯(薬)学総合研究科, 特任教授 (70183176)
• Co-Investigator(Kenkyū-buntansha): IKUTA Kazuyoshi 大阪大学, 微生物研究所, 教授 (60127181) ; NAKAYA Takaaki 大阪大学, 微生物研究所, 特任准教授 (80271633) ; NAKAMURA Shota 大阪大学, 微生物研究所, 助教 (90432434)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: ウイルス / HIV / 感染症 / エイズ / 進化 / アフリカ / コンゴ盆地 / コンゴ民主共和国

Research Abstract

The purpose of this study is to clarify the mystery of AIDS pandemic (when, where and how did it occur?) as well as the origin of HIV. To do so, we have expedited into far remote provinces in both Democratic Republic of Congo and Republic of Congo. In addition to the conventional molecular epidemiological techniquues, we also employed the so-called second generation high-throughput DNA sequencer. So far the areas showing the most diversified profiles of HIV genotypes are the central region of Congo basin and nearby areas the majority of which is covered with the giant Congo River and its numerous branch rivers. In those areas, not only a great variety of genotypes of HIV strains are co-circulating but also new types of viruses seem to be continuously generated with high probability by way of dual infection of two different genotypic HIV strains and recombination of their genomes as a natural consequent event.

Research Products

• [Journal Article] Joint research project on infectious diseases in West African subregion (2014)
  • Author(s): Ido E, Suzuki T, Ampofo WK, Ayi I, Yamaoka S, KoramKA, Ohta N
  • Journal Title: J Disaster Res Volume: (in press)
  • Peer Reviewed
• [Journal Article] Impact of amino acid substitutions in the V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 on viral neutralization susceptibility to broadly neutralizing antibodies specific for the CD4 binding site (2014)
  • Author(s): Utachee P, Isarangkura-na-ayuthaya P, Tokunaga K, Ikuta K, Takeda N, Kameoka M
  • Journal Title: Retrovirology Volume: 11 Pages: 32
  • Peer Reviewed
• [Journal Article] Characterization of human immunodeficiency virus type 1 CRF01_AE env genes derived from recently infected Thai individuals (2014)
  • Author(s): Chaitaveep N, Utachee P, Nakamura S, Chuenchitra T, Ekpo P, Takeda N, Pattanapanyasat K, Kameoka M
  • Journal Title: Microbes Infect Volume: 16(2) Pages: 142-152
  • Peer Reviewed
• [Journal Article] Establishment of in-house quantitative real-time RT-PCR assay for HIV-1 viral load measurement and its application to evaluate efficacy of ART in Ghanaian patients in an urban setting (2014)
  • Author(s): Barnor JS, Yamamoto N, Brandful JAM, Ampofo W, Bonney JHK, Bonney E, Odoom JK, Aidoo S, Alale M, Ntim NA, Amoah YO, Ofori SB, Ndzinu J, Aziati ID, Addo, NA, Nyarko A, Ido E, Ishikawa K, Yamaoka S :
  • Journal Title: J AIDS Clinic Res Volume: 5 Pages: 305.1000305
  • Peer Reviewed
• [Journal Article] 東京医科歯科大学がガーナ大学野口医学記念研究所で展開している2つの感染症プロジェクト (2013)
  • Author(s): 井戸栄治, 山岡昇司
  • Journal Title: ウイルス Volume: 63 Pages: 79-86
  • Peer Reviewed
• [Journal Article] Variables influencing anti-human immunodeficiency virus type 1 neutralizing human monoclonal antibody (NhMAb) production among infected Thais (2013)
  • Author(s): Akapirat S, Avihingsanon A, Ananworanich J, Schuetz A, Ramasoota P, Luplertlop N, Ono K, Ikuta K, Utachee P, Kameoka M, Leaungwutiwong P
  • Journal Title: Southeastern Asian J Trop Med Public Health Volume: 44(5) Pages: 825-841
  • Peer Reviewed
• [Journal Article] A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads (2013)
  • Author(s): Sakudo A, Ikuta K
  • Journal Title: Int J Mol Med Volume: 30(2) Pages: 437-442
  • Peer Reviewed
• [Journal Article] Discovery of novel low-molecularweight HIV-1 inhibitors interacting with cyclophilin A using in silico screening and biological evaluations (2013)
  • Author(s): Tian YS, Verathamjamras C, Kawashita N, Okamoto K, Yasunaga T, Ikuta K, Kameoka M, Takagi T
  • Journal Title: J Mol Medels Volume: 19(1) Pages: 465-475
  • Peer Reviewed
• [Journal Article] Misinterpretation in virus clearance studies of biological products due to an uncommon discrepancy between cytopathic effects and infectivity of human immunodeficiency virus (HIV) (2013)
  • Author(s): Urayama T, Cameron R, Sato T, Yunoki M, Ikuta K
  • Journal Title: Biologicals Volume: 41(2) Pages: 125-127
  • Peer Reviewed
• [Journal Article] Diagnosis of HIV-1 infection by near-infrared spectroscopy : Analysis using molecular clones of various HIV-1 subtypes (2012)
  • Author(s): Sakudo A, Suganuma Y, Sakima R, Ikuta K
  • Journal Title: Clin Chim Acta Volume: 413 Pages: 467-472
  • Peer Reviewed
• [Journal Article] Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009-2010 (2012)
  • Author(s): Yasugi M, Nakamura S, Daidoji T, Ramadhany R, Yang CS, Yasunaga T, Iida T, Horii T, Ikuta K, Takahashi K, Nakaya T
  • Journal Title: PLoS One Volume: 7 Pages: e30946
  • Peer Reviewed
• [Journal Article] Metagenomic analysis of bacterial infections by means of high-throughput DNA sequencing (2011)
  • Author(s): Nakamura S, Nakaya T, Iida T
  • Journal Title: Exp Biol Med Volume: 236(8) Pages: 968-971
  • Peer Reviewed
• [Journal Article] Viral factors involved in adaptor-related protein complex 2 alpha 1 subunit-mediated regulation of human immunodeficiency virus type 1 replication (2011)
  • Author(s): Sapsutthipas S, Kitagawa Y, Tokunaga K, Ikuta K, Kameoka M
  • Journal Title: Southeastern Asian J Trop Med Public Health Volume: 42 Pages: 311-319
  • Peer Reviewed
• [Journal Article] Molecular evolution of HIV-1 CRF01_AE Env in Thai patients (2011)
  • Author(s): Boonchawalit S, Jullaksorn D, Uttiyoung J, Yowang A, Krathong N, Chautrakul S, Yamashita A, Ikuta K, Roobsoong A, Kanitvittaya S, Sawanpanyalert P, Kameoka M
  • Journal Title: PLoS One Volume: 6 Pages: e27098
  • Peer Reviewed
• [Journal Article] High frequency of HIV-1 dual infections in Cameroon, West Central Africa (2011)
  • Author(s): Ndembi N, Iwamoto S, Ngansop C, Lemey P, Abimiku A, Mbanya D, Kaptue L, Ido E
  • Journal Title: J Acquir Immune Defic Syndr Volume: 57(2) Pages: e25-e27
  • Peer Reviewed
• [Journal Article] Design and evaluation of antiretroviral peptides corresponding to the C-terminal heptad repeat region (C-HR) of human immunodeficiency virus type 1 envelope glycoprotein gp41 (2010)
  • Author(s): Soonthornsata B, Tian YS, Utachee P, Sapsutthipas S, Isarangkura-na-Ayuthaya P, Auwanit W, Takagi T, Ikuta K, Sawanpanyalert P, Kawashita N, Kameoka M :
  • Journal Title: Virology Volume: 405(1) Pages: 157-164
  • Peer Reviewed
• [Presentation] コンゴ民主共和国北キヴ州に流行するHIV のサブタイプ解析 (2013)
  • Author(s): 井戸栄治, Stormy Karhemere, Joseph Kayumba, Joachim-Massa Mukedi, Ishmael Aziati, Esinam Agbosu, William Ampofo, 伊 吹謙太郎, 山岡昇司, Jean-Jacques Muyembe
  • Organizer: 第54回日本熱帯医学会大
  • Place of Presentation: 長崎
  • Year and Date: 20131003-05
• [Presentation] Molecular epidemiology of HIV in the Congo basin (Democratic Republic of Congo) analyzed by high-throughput DNA sequencing (2013)
  • Author(s): Ido E, Karhemere S, Nakamura S, Nakaya T, Barnor J, Brandful J, Ampofo W, Aziati I, Agbosu E, Ebengho M, Bakuba K, Ikuta K, Yamaoka S, Muyembe-Tamfum JJ
  • Organizer: 6eme Congres International De Pathologie Infectieuse et Parasitaire (CIPIP)
  • Place of Presentation: Kinshasa, DR Congo
  • Year and Date: 20130613-15
• [Presentation] High-throughput DNA sequencing is a powerful tool to analyze dual and multiple HIV infections : its first application to the study in DRC (2013)
  • Author(s): Ido E, Karhemere S, Ebengho M, Nakamura S, Nakaya T, Brandful J, Barnor J, Ampofo W, Yamaoka S, Muyembe JJ
  • Organizer: 20th Conference on Retroviruses and Opportunistic Infections
  • Place of Presentation: Atlanta, USA
  • Year and Date: 20130303-06
• [Presentation] 次世代型高速シーケンサーはHIV重感染検体の解析に威力を発揮する−コンゴ民主共和国におけるHIV の分子疫学研究での適用例から (2012)
  • Author(s): 井戸栄治, Stomy Karhemere, Max Ebengho, 中村昇太, 中屋隆明, Ishmael Aziati, James Brandful, Jacob Barnor, William Ampofo, 生田和良, 山岡昇司, Jean-Jacques Muyembe
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20121113-15
• [Presentation] カメルーン共和国北西部におけるHIV の分子疫学 (2011)
  • Author(s): 井戸栄治, Nicaise N, 岩元静香, Charlotte N, Philippe L, Alashle A, Mbaya D, Kaptue LK
  • Organizer: 第25回日本エイズ学会学術集
  • Place of Presentation: 東京
  • Year and Date: 20111130-1202
• [Presentation] コンゴ盆地におけるHIV の分子疫学-エイズウイルス生成の現場から (2011)
  • Author(s): 井戸栄治、Karhemere S、Taty-Taty R、多田哲子、安永みゆき、岩元静香、梅原 綾、Parra HJ、Kashongwe Z、Muyembe JJ
  • Organizer: 第52回日本熱帯医学大会・第26回日本国際医療保健学会学術大会・合同大会
  • Place of Presentation: 東京
  • Year and Date: 20111104-06
• [Presentation] Study on the evolution of HIV-1 envelope gene in Thai patients (2011)
  • Author(s): Kameoka M, Boonchawalit S, Jullaksorn D, Uttiyoung J, Yowang A, Krathong N, Chautrakul S, Ikuta K, Roobsoong A, Kanitvittaya S, Sawanpanyalert P
  • Organizer: XVth International Congress of Virology (IUMS 2011)
  • Place of Presentation: Sapporo, Japa
  • Year and Date: 20110911-16
• [Presentation] High frquency of HIV-1 dual infections in central African countries (2011)
  • Author(s): Ido E, Ndembi N, Taty-Taty R, Iwamoto S, Tada T, Brandful J, Karhemere S, MuyembeJJ
  • Organizer: XVth International Congress of Virology (IUMS 2011)
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110911-16
• [Presentation] Establishment of in-house quantitative rea-time RT PCR assay for monitoring for HIV-1 viral loads and efficacy evaluation of ART in Ghanaian AIDS patients in an urban setting (2011)
  • Author(s): Barnor J, Brandful J, Ampofo W, Yamamoto N, Bonney K, Bonney M, Alale M, Ntim A, AmoahY, fori S, Addo A, Nyarko A, Ido E, Ishikawa K, Yamaoka S
  • Organizer: 6^<th> IAS Conferenceon HIV Pathogenesis, Treatment and Prevention
  • Place of Presentation: Rome, Italy
  • Year and Date: 20110717-20
• [Presentation] Molecular epidemiological study in Democratic Republic of Congo : Double and even triple infections of different genotypic HIV-1 strains are not rare events (2011)
  • Author(s): Ido E, Karhemere S, Ahuka S, Omokoko M, Tada T, Iwamoto S, Dauly N, Imani M, Kashongwe Z, Muyembe JJ
  • Organizer: Keystone Symposia Conference-HIV Evolusion, Genomics and Pathogenisis (X7)
  • Place of Presentation: Whistler, Canada
  • Year and Date: 20110320-25
• [Presentation] コンゴ民主共和国赤道州におけるHIV の分子疫学 (2010)
  • Author(s): 井戸栄治, Max Ebengho, 岩 元静香, Stormy Karhemere, Ehungu Gini, Jean-Jacques Muyembe
  • Organizer: 第24回日本エイズ学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20101124-26
• [Presentation] 次世代シークエンサーを用いた臨床検体(血清)のメタゲノミック診断 (2010)
  • Author(s): 中屋隆明, 中村 昇太, 飯田 哲也, 堀井俊宏,柚木 幹弘, 生田 和良, 岡本 仁子,永井 美之 :
  • Organizer: 第58回日本ウイルス学会学術集
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Book] メタゲノム解析技術の最前線 (2011)
  • Author(s): 中村昇太,中屋隆明,飯田哲也
  • Publisher: (株) シーエムシー出版