2012 Fiscal Year Final Research Report
Establishment of a macaque cancer model using cancer cells derived from MHC homozygous macaque iPS cells
| Project/Area Number |
22300143
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Yasushi 滋賀医科大学, 医学部, 准教授 (90324566)
ISHIGAKI Hirohito 滋賀医科大学, 医学部, 助教 (90432301)
TORII Ryuuzou 滋賀医科大学, 動物生命科学研究センター, 教授 (50106647)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIINA Takashi 東海大学, 医学部, 准教授 (00317744)
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| Project Period (FY) |
2010 – 2012
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| Keywords | iPS 細胞 / カニクイザル / 癌 / MHC |
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| Research Abstract |
We made somatic cells from iPS cells derived from cynomolgus macaque, which homozygously had a certain haplotype of major histocompatibility complex (Mafa-HT1). And then, we transduced oncogenes to the differentiated cells resulting in establishment of malignant cells, an embryonal carcinoma PTY and a glioblastoma NPC#5PR2. These two cancer cell lines could make tumors in NOG mouse. We transplanted PTY cellsinto subcutaneous, ovary and abdominal cavity of a cynomolgus macaque with heterogygous MHC. However, all PTY cells were rejected after 4 weeks post transplantation. On the other hand, we are transplanting NPC#5PR2 cells into an immune privilege site, brain, of a Mafa HT-1 heterozygous monkey.
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[Journal Article] Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in ynomolgus monkeys carrying Mafa-A*052:022012
Author(s)
Arikata M, Itoh Y, Okamatsu M, Maeda T, Shiina T, Tanaka K, Suzuki S, Nakayama M, Sakoda Y, Ishigaki H, Takada A, Ishida H, Soda K, Pham VL, Tsuchiya H, Nakamura S, Torii R, Shimizu T, Inoko H, Ohkubo I, Kida H, Ogasawara K
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Journal Title
PLos ONE
Volume: 7
Pages: e37220
DOI
Peer Reviewed
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