The control strategy of stress-induced UCP2 expression and ROS generation in diabetes mellitus

2012 Fiscal Year Final Research Report

• Project/Area Number: 22500767
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyoto Prefectural University
• Principal Investigator: MINAMIYAMA Yukiko 京都府立大学, 大学院・生命環境科学研究科, 教授 (00362989)
• Co-Investigator(Kenkyū-buntansha): ICHIKAWA Hiroshi 同志社大学, 生命医科学部, 教授 (60336732) ; TAKEMURA Shigekazu 大阪市立大学, 医学研究科, 講師 (00322363)
• Project Period (FY): 2010 – 2012
• Keywords: 生活習慣病 / ミトコンドリア機能 / 低鉄 / 抗酸化物質 / 食事制限 / UCP2

Research Abstract

It was found that the enhancement of oxidative stress by mitochondrial dysfunction and over-expression of UCP2 that negatively regulates reactive oxygen species (ROS) from mitochondria in diabetes mellitus.Calorie restrictions improved the diabetic complications.Furthermore, we found that iron deposition due to abnormal iron metabolism in diabetic tissues.It has been clear that the removal of iron therapy is effective in the treatment of diabetes. Thus, phlebotomy treatments currently being conducted in chronic hepatitis C treatment can be expected as an effective treatmentas an applicationto diabetes, in developing countries, particularly. In addition, antioxidants are also effective. S-allyl cysteine that is an antioxidantcomponent of the aged garliccould improve diabetic condition by controlling the mechanisms related to lipid metabolism and glucose metabolism.

Research Products

• [Journal Article] Comparison of in vivo and in vitro antioxidative parameters for eleven food factors (2013)
  • Author(s): HP. Indo, I. Nakanishi, K. Ohkubo, Hsiu-Yen. Chuan, M. Nyui, S. Manda, K. Matsumoto, K. Fukuhara, K. Anzai, N. Ikota, H. Matsui, Y. Minamiyama, A. Nakajima, H. Ichikawa, S. Fukuzumi, T. Ozawa, C. Mukai, and HJ. Majima
  • Journal Title: The Royal Society of Chemistry Volume: 3 Pages: 4535-4538
  • Peer Reviewed
• [Journal Article] Suppression of indomethacin-induced apoptosis in the small intestine due to Bach1 deficiency (2011)
  • Author(s): A. Harusato, Y. Naito, T. Takagi, K. Uchiyama, K. Mizushima, Y. Hirai, S. Yamada, T. Tuji, H. Yoriki, R. Horie, K. Inoue, K. Fukumoto, O. Handa, T. Ishikawa, S. Kokura, Y. Minamiyama,H. Ichikawa, A. Muto, K. Igarashi, T. Yoshikawa.
  • Journal Title: Free Radic Res Volume: 45(6) Pages: 717-27
  • Peer Reviewed
• [Journal Article] Iron restriction improves type II diabetes mellitus of Otsuka Long-Evans Tokushima Fatty (OLETF) rats via inhibition of endogenously reactive oxygen species and the regulation of pancreas peroxisome proliferator-activated receptor-delta (PPAR-delta) and hypoxia inducible factor (HIF)-1alpha. (2010)
  • Author(s): Y. Minamiyama,S. Takemura, S. Kodai, H. Shinkawa, T. Tsukioka, Y. Naito, H. Ichikawa, T. Yoshikawa, S. Okada
  • Journal Title: Am J Physiol Volume: 298(6) Pages: E1140-9
  • Peer Reviewed
• [Journal Article] Coadministration of carvedilol attenuates nitrate tolerance by preventing cytochrome p450 depletion. (2010)
  • Author(s): A. Nakahira, Y. Minamiyama, S. Takemura,H. Hirai, Y. Sasaki, S. Okada, Y. Funae, S. Suehiro
  • Journal Title: Circ J Volume: Aug;74(8) Pages: 1711-7
  • Peer Reviewed
• [Journal Article] Generation of reactive oxygen species in sperms of rats as an earlier marker for evaluating the toxicity of endocrine-disrupting chemicals. (2010)
  • Author(s): Y. Minamiyama, H. Ichikawa, S. Takemura, H. Kusunoki, Y. Naito, T. Yoshikawa
  • Journal Title: Free Radic Res Volume: 44(12) Pages: 1398-406
  • Peer Reviewed
• [Journal Article] S-allyl cysteine improves nonalcoholic fatty liver disease in type 2 diabetes Otsuka Long-Evans Tokushima Fatty rats via regulation of hepatic lipogenesis and glucose metabolism
  • Author(s): S. Takemura, Y. Minamiyama, S. Kodai, H. Shinkawa, T. Tsukioka, S. Okada, H. Azuma, S. Kubo
  • Journal Title: J. Clin. Biol. Nutr. Volume: (in press)
  • Peer Reviewed
• [Presentation] Effects of Iron Restriction on Hepatic Mitochondrial Iron Metabolism and Oxidative Stress in Nonalcoholic Fatty Liver Disease (NAFLD) in Otsuka Long-Evans Tokushima Fatty (OLETF) Rats (2013)
  • Author(s): Y. Minamiyama, S. Takemura, S. Taketani, Y. Naito, H. Ichikawa,T. Takagi, S. Okada, and T. Yoshikawa
  • Organizer: Digestive Disease Week 2013
  • Place of Presentation: Orland, FL
  • Year and Date: 20130518-22
• [Presentation] Attenuation of nonalcoholic fatty liver disease (NAFLD) in type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats via regulation of hepatic lipogenesis and glucose metabolism by administration of S-allyl cysteine (2013)
  • Author(s): Takemura S, Minamiyama Y, Kodai S, Shinkawa H, Kubo S
  • Organizer: Digestive Disease Week 2013
  • Place of Presentation: Orland, FL
  • Year and Date: 20130518-22
• [Presentation] Generation of Reactive Oxygen Species from the Rat Sperm Mitochondria is an Excellent Marker for the Biological Toxicity of Endocrine-Disrupting Chemicals (2012)
  • Author(s): H. Ichikawa, Y. Minamiyama, T. Takagi, O. Handa, Y. Naito, T. Yoshikawa
  • Organizer: 16th Biennial Meeting of the Society for Free Radical Research (International SFRR 2012)
  • Place of Presentation: London, UK
  • Year and Date: 20120906-09
• [Presentation] Iron Metabolism of Hepatic Mitochondria and Oxidative Stress in Type 2 Diabetes Mellitus in Otsuka Long-Evans Tokushima Fatty (OLETF) Rats (Effect of Iron Depletion) (2012)
  • Author(s): Y. Minamiyama, S. Takemura, S. Taketani, Y. Naito, H. Ichikawa, K. Misumi. S. Okada, and T. Yoshikawa
  • Organizer: 16th Biennial Meeting of the Society for Free Radical Research (International SFRR 2012)
  • Place of Presentation: London, UK
  • Year and Date: 20120906-09
• [Presentation] Effects of alpha-tocopherol and gamma-tocopherol on diabetic complications in type II diabetic rats. (2011)
  • Author(s): Y. Minamiyama, S. Takemura, H. Ichikawa, S. Okada, Y. Naito and T. Yoshikawa
  • Organizer: International Conference on Food Factors 2011
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 20111120-23
• [Presentation] The role of histidine on wound healing using cultured rat intestinal epithelial. (2011)
  • Author(s): H. Ichikawa,Y. Minamiyama
  • Organizer: International Conference on Food Factors 2011
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 20111120-23
• [Presentation] The role of histidine on wound healing using cultured rat intestinal epithelial cells. (2011)
  • Author(s): K. Hori, H. Ichikawa,T. Takagi, Y. Minamiyama, M. Nakamura, A. Doi, Y. Kawabata, T. Kaneda, K. Uchiyama, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 20110801-0904
• [Presentation] The histidine affects delayed wound healing induced by indomethacin using cultured rat epithelial cells. (2011)
  • Author(s): S. Matsuo, H. Ichikawa, Y. Minamiyama, T. Takagi, R. Fuchiue, A. Emoto, M. Suetsugu, K. Uchiyama, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 20110801-0904
• [Presentation] The effect of Kampo Medicine "Dai-kenchu-to" on wound healing of rat intestinal epithelial cells. (2011)
  • Author(s): H. Asai, H. Ichikawa, Y. Minamiyama, T. Takagi, A. Wada, Y. Ozaki, K. Okazaki, C. Minami, O. Handa, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 20110801-0904
• [Presentation] Sperm dysfunction from aging is involved in the oxidative stress. (2011)
  • Author(s): R. Yamamoto, Y. Minamiyama, H. Ichikawa, T. Takagi, A. Ishii, Y. Sato, E. Miyanishi, M. Yasui, O. Handa, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 20110801-0904
• [Presentation] Generation of reactive oxygen species from the sperm mitochondria of rats is an excellent marker for the biological toxicity of endocrine-disrupting chemicals. (2011)
  • Author(s): E. Miyanishi, Y. Minamiyama, IchikawaH, T. Takagi, A. Ishii, O. Handa, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 20110801-0904
• [Presentation] Protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells - involvement of heat shock protein 70 and reactive oxygen species. (2011)
  • Author(s): O. Handa, Y. Naito, A. Fukui, Y. Qin, Y. Uehara, T. Tsuji, H. Yoriki, R. Horie, K. Katada, K. Kamada, K. Uchiyama, T. Ishikawa, T. Takagi, Y. Minamiyama, H. Konishi, N. Yagi, S. Kokura, H. Ichikawa, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 20110801-0904
• [Presentation] Calorie restriction improves diabetic complications via reduction of mitochondrial reactive oxygen species in Type II diabetic rats. (2011)
  • Author(s): Y. Minamiyama, S. Takemura,H. Ichikawa, S. Okada and T. Yoshikawa
  • Organizer: 4th INTERNATIONAL SYMPOSIUM NUTRITION, OXYGEN BIOLOGY, AND MEDICINE
  • Place of Presentation: Paris, France
  • Year and Date: 20110615-17
• [Presentation] Generation of reactive oxygen species from the sperm mitochondria of rats is an excellent marker for the biological toxicity of endocrine-disrupting chemicals. (2010)
  • Author(s): Y. Minamiyama, H. Ichikawa, S. Takemura, Y. Naito, T. Yoshikawa
  • Organizer: 7thconference of Asian Society for Mitochondrial Research and Medicine (ASMRM) and 10thconference of Japanese Society for Mitochondrial Research and Medicine (J-mit)
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20101216-18
• [Presentation] Effects of aging on sperm function and oxidative stress. (2010)
  • Author(s): Y. Minamiyama, H. Ichikawa, TakemuraS, T. Kawagishi, A. Wakahara, C. Minami, M. Yasui, R. Yamamoto, NaitoY, T. Yoshikawa
  • Organizer: 15th Biennial Meeting of the Society for Free Radical Research (International SFRR 2010)
  • Place of Presentation: Orland, FL
  • Year and Date: 20101117-21
• [Book] 酸化ストレスとAOB 第2版 (2011)
  • Author(s): 岡田茂,南山幸子
  • Total Pages: 105
  • Publisher: マイクロ出版