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2012 Fiscal Year Final Research Report

Development of probiotics for preventing opportunistic infections of fish

Research Project

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Project/Area Number 22580211
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNihon University

Principal Investigator

SUGITA Haruo  日本大学, 生物資源科学部, 教授 (50139052)

Project Period (FY) 2010 – 2012
Keywordsプロバイオティクス / 魚類 / 腸内細菌叢 / AHL / 日和見感染症 / 16S rRNA遺伝子
Research Abstract

The intestinal tracts of ayu (Plecoglossus altivelis), Japanese smelt (Hypomesus nipponensis) and goldfish (Carassius auratus) were colonized with the AHL-producing aeromonads. On the other hand, members of genus Shewanella were isolated as AHL-decomposer from the intestinal tract of goldfish. These results suggest that the AHL-decomposing Shewanella seems to be one of the candidate of probiont for preventing aneromonasis of freshwater fish.

  • Research Products

    (28 results)

All 2013 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (18 results) Book (2 results)

  • [Journal Article] ヒイラギ腸管内の硫酸還元細菌について.2012

    • Author(s)
      陳家輝、相良和之、糸井史朗、杉田治男
    • Journal Title

      水産増殖

      Volume: 60巻 Pages: 519-521

    • Peer Reviewed
  • [Journal Article] クローンライブラリー法による沿岸魚類腸内細菌群集の解析2012

    • Author(s)
      田中祐輔、陳家輝、小崎照邦、込宮雄介、糸井史朗、杉田治男
    • Journal Title

      水産増殖

      Volume: 60巻 Pages: 333-340

    • Peer Reviewed
  • [Journal Article] Effect of the C-terminal domain of Vibrio proteolyticus chitinase A on the chitinolytic activity in association with pH changes.2012

    • Author(s)
      S. Itoi, Y. Kanomata, S. Uchida, K. Kadokura, T. Nishio, T. Oku, H. Sugita
    • Journal Title

      Letters inApplied Microbiology

      Volume: 54巻 Pages: 441-446

    • Peer Reviewed
  • [Journal Article] Bacterial diversity of the upper and lower intestines of the common carp (Cyprinus carpio Linnaeus, 1758) reared in an indoor tank.2012

    • Author(s)
      H. Sugita, H. Mizuki
    • Journal Title

      Journal of AppliedIchthyology

      Volume: 28巻 Pages: 647?648

    • DOI

      DOI:10.1111/j.1439-0426.2012.01965.x

    • Peer Reviewed
  • [Journal Article] 養殖ワカサギ腸内細菌のN-アシルホモセリンラクトン生産能.2012

    • Author(s)
      陳家輝,小川真幸,相良和之,糸井史朗,杉田治男
    • Journal Title

      日水誌

      Volume: 78巻 Pages: 259-261

    • Peer Reviewed
  • [Journal Article] Diversity of siderophore-producing bacteria isolated from the intestinal tracts of fish along the Japanese coast.2012

    • Author(s)
      H. Sugita, H. Mizuki, S. Itoi
    • Journal Title

      Aquaculture Research

      Volume: 43巻 Pages: 481-488

    • DOI

      DOI:10.1111/j.1365-2109.2011.02851.x

    • Peer Reviewed
  • [Journal Article] 養殖アユ腸管から分離されたN-アシルホモセリンラクトン生産菌.2011

    • Author(s)
      小川真幸、陳家輝、糸井史朗、杉田治男
    • Journal Title

      水産増殖

      Volume: 59巻 Pages: 595-600

    • Peer Reviewed
  • [Journal Article] Culturable bacterial flora in the intestinal tract of Japanese pufferfish Takifugu rubripes.2010

    • Author(s)
      H. Sugita, K. Sugiyama, S. Itoi
    • Journal Title

      Aquaculture Science

      Volume: 58巻 Pages: 437-438

    • Peer Reviewed
  • [Presentation] 海洋性乳酸菌Lactococcus lactissubsp.lactisのトラフグ腸管への定着性に関する研究.2013

    • Author(s)
      高梨志保里、宮之本昴也、糸井史朗、杉田治男.
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2013-03-29
  • [Presentation] Vibrio科細菌検出プライマーの有効性.2013

    • Author(s)
      相良和之、陳家輝、糸井史朗、杉田治男.
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京).
    • Year and Date
      2013-03-27
  • [Presentation] Sulfate-reducing bacteria in the intestinal tract of spotnape ponyfish Leiognathus nuchalis. 10th Japan-Korea2012

    • Author(s)
      K. Sagara, C. H. Chen, S. Itoi, H. Sugita.
    • Organizer
      Korea-Japan Joint Symposium on Aquaculture 2012
    • Place of Presentation
      Nagasaki.
    • Year and Date
      2012-12-08
  • [Presentation] Colonization of Lactococcus lactis subsp. lactis strains in the intestinal tract of goldfish.2012

    • Author(s)
      J. Uchida, A. Miura, S. Itoi, H.Sugita.
    • Organizer
      The 9th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Kochi, Japan.
    • Year and Date
      2012-07-14
  • [Presentation] Microbial communities in intestinal tracts of Japanese coastal fish.2012

    • Author(s)
      C.H. Chen, Y. Tanaka, K. Sagara, S. Itoi, H.Sugita.
    • Organizer
      The 9th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Kochi, Japan.
    • Year and Date
      2012-07-13
  • [Presentation] I腸内細菌研究の現状認識.3.魚類の腸内細菌叢.シンポジウム:水産「プロバイオティクス」の創成2012

    • Author(s)
      杉田治男.
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京).
    • Year and Date
      2012-03-30
  • [Presentation] 乳酸菌Lactococcus lactis subsp.lactisの海水魚類腸管への定着性.2012

    • Author(s)
      三浦愛、糸井史朗、杉田治男
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2012-03-28
  • [Presentation] 沿岸魚類の腸内細菌群集について.2012

    • Author(s)
      陳家輝、田中祐輔、相良和之、糸井史朗、杉田治男.
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2012-03-28
  • [Presentation] Possibility of marine Lactococcus lactis subsp.lactis strain as probiotic for the pufferfish Takifugu rubripes.2011

    • Author(s)
      A. Miura, S. Takanashi, S. Itoi, H. Sugita.
    • Organizer
      The International Symposium on Muscle Biochemistry, University of Tokyo
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-10-28
  • [Presentation] Advantage ofchitin oligosaccharides utilization in Lactococcus lactis strain from marine environment.2011

    • Author(s)
      S. Takanashi, S. Itoi, H. Sugita.
    • Organizer
      The International Symposium on Muscle Biochemistry University of Tokyo
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-10-28
  • [Presentation] 由来の異なるLactococcus lactis subsp.lactisの宿主選択性.2011

    • Author(s)
      三浦愛、糸井史朗、杉田治男.
    • Organizer
      平成23年度日本水産学会秋季大会
    • Place of Presentation
      長崎大学(長崎).
    • Year and Date
      2011-10-01
  • [Presentation] Sulphate-reducing bacteria in the eelgrass bed of the Izu Peninsula2011

    • Author(s)
      C.H. Chen, Y. Tanaka, T. Kozaki, S. Itoi, K.Yoshihara, H. Sugita.
    • Organizer
      Japan. IUMS2011
    • Place of Presentation
      Sapporo.
    • Year and Date
      2011-09-07
  • [Presentation] Microflora of the intestinal tracts of fish along the Japanese coast.2011

    • Author(s)
      Y. Tanaka, C.H. Chen, T. Kozaki, Y. Komiya,S. Itoi, H. Sugita.
    • Organizer
      IUMS2011
    • Place of Presentation
      Sapporo.
    • Year and Date
      2011-09-07
  • [Presentation] クローンライブラリィ法による沿岸魚類腸内細菌叢の解析.2011

    • Author(s)
      田中祐輔、陳家輝、小崎輝邦、糸井史朗、杉田治男.
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2011-03-30
  • [Presentation] Microbial communities in the intestinal tracts of coastal fish.2010

    • Author(s)
      Y. Tanaka, C.H. Chen, T. Kozaki, S. Itoi, H.Sugita.
    • Organizer
      2010Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
    • Place of Presentation
      Busan, Korea.
    • Year and Date
      2010-11-13
  • [Presentation] The AHL-producing bacteria in the intestinal tracts of ayu Plecoglossus altivelis.2010

    • Author(s)
      M. Ogawa, S. Itoi, H. Sugita.
    • Organizer
      2010Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
    • Place of Presentation
      Busan, Korea.
    • Year and Date
      2010-11-13
  • [Presentation] Microbial communities in the eelgrass bed of the Izu Peninsula2010

    • Author(s)
      C.H. Chen, T. Kozaki, Y. Tanaka, S. Itoi, K.Yoshihara, H. Sugita.
    • Organizer
      Japan.2010 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
    • Place of Presentation
      Busan, Korea.
    • Year and Date
      2010-11-13
  • [Presentation] Characterization of amarine lactic acid bacterium Lactococcus lactis subsp. lactis isolated from the pufferfish.2010

    • Author(s)
      S. Itoi, H. Sugita.
    • Organizer
      9^<th>International Marine BiotechnologyConference
    • Place of Presentation
      Quigdao, China.
    • Year and Date
      2010-10-10
  • [Book] 海の環境微生物学2011

    • Author(s)
      石田祐三郎・杉田治男
    • Total Pages
      249
    • Publisher
      恒星社厚生閣
  • [Book] 食品微生物学辞典2010

    • Author(s)
      杉田治男
    • Total Pages
      341

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Published: 2014-08-29  

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