2012 Fiscal Year Final Research Report
Enhanced safety of infertile therapy by means of quality control of sperm - relationship between vacuole in sperm head and DNA fragmentation
| Project/Area Number |
22591837
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
KANEKO Satoru 東京歯科大学, 歯学部, 講師 (40214457)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 品質管理 / 精子頭部空胞 / DNA 損傷 / 顕微授精 / 染色体 |
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| Research Abstract |
The early stage of DNA fragmentation in a single nucleus could be observed by single cell pulse field gel electrophoresis (SCPFGE). First, a few large fibrous fragments were derived from a bundle of long chain fibers, the cleavages were advanced until finally almost all the DNA was shredded to granular fragments. There found no significant correlation between DNA fragmentation and vacuole in sperm head. The cell culture under high oxygen concentration caused increment of the percentage of DNA fragmentation.
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