The serum glycoprotein/peptide analysis for discovery of novel biomarkers of hepatocellular carcinoma.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790521
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: SOGAWA Kazuyuki 千葉大学, 医学部・附属病院, 寄附研究部門教員 (50436440)
• Project Period (FY): 2010 – 2011
• Keywords: プロテオミクス / 糖タンパク質 / 糖ペプチド

Research Abstract

Changes in glycosylation, most notably fucosylation, have been associated with the development of hepatocellular carcinoma (HCC). In this report, the levels of growth/differentiation facter 5 (GDF5) was analyzed individually with currently used marker, AFP and PIVKA-II, for the ability to distinguish between a diagnosis of cirrhosis and HCC. This analysis was done on serum from 50 patients with cirrhosis and 50 serum samples from patients with cirrhosis plus HCC. The levels of GDF5 was significantly higher in patients with HCC compared with those with cirrhosis (P<0.01).

Research Products

• [Journal Article] Increased serum levels of pigment epithelium-derived factor by excessive alcohol consumption-Detection and identification by a three-step serum proteome analysis- (2011)
  • Author(s): Sogawa K, Kodera Y, Satoh M, Kawashima Y, Umemura H, Maruyama K, Takizawa H, Yokosuka O, Nomura F
  • Volume: Vol.35、No.2
  • Pages: 211-217
  • Peer Reviewed
• [Journal Article] The measurement of a fibrinogen alpha C-chain 5.9 kDa fragment (FIC 5.9) using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution、 Clinica Chimica Acta (2011)
  • Author(s): Sogawa K, Kodera Y, Noda K, Ishizuka Y, Yamada M, Umemura H, Maruyama K, Tomonaga T, Yokosuka O, Nomura F
  • Volume: Vol.412 、No.11-12
  • Pages: 1094-1099
  • Peer Reviewed
• [Journal Article] Fibrinogen alpha C-chain 5.9kDaフラグメント(FIC5.9)の線維化マーカーとしての評価 (2011)
  • Author(s): 曽川一幸, 野田健太, 梅村啓史, 清宮正徳, 金井文彦, 今関文夫, 瀧澤弘隆, 横須賀收, 野村文夫
  • Journal Title: 医学と薬学 Volume: 66巻 Pages: 329-337
  • Peer Reviewed
• [Presentation] HPLC とMALDI-TOF MSによる血清糖鎖欠損トランスフェリンの評価 (2012)
  • Author(s): 曽川一幸, 飯田史枝, 佐藤守, 川島祐介, 山田真子, 角谷真知子, 丸山勝也, 和田芳直, 野村文夫
  • Organizer: 第31回アルコール医学生物学研究会学術集会
  • Place of Presentation: ホテル日航金沢
  • Year and Date: 2012-01-28
• [Presentation] 血清糖鎖欠損トランスフェリンの高感度測定を目指して (2010)
  • Author(s): 曽川一幸, 飯田史枝, 佐藤守, 川島祐介, 山田真子, 角谷真知子, 丸山勝也, 和田芳直, 野村文夫
  • Organizer: 第30回アルコール医学生物学研究会学術集会
  • Place of Presentation: 久留米翠香園ホテル
  • Year and Date: 2010-11-26
• [Presentation] Measurement of 5.9 kDa fragment of fibrinogen alpha-C chain (FIC 5.9) by MALDI-TOF MS with stable-isotope labeled standard in patients with liver diseases (2010)
  • Author(s): Sogawa K, Kodera Y, Noda K, Umemura H, Matsushita K, Kojima R, Katayama K, Nomura F
  • Organizer: HUPO 9th Annual World Congress
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-20
• [Presentation] 消化器領域における多戦略的疾患プロテオミクス (2010)
  • Author(s): 曽川一幸, 高野重紹, 清宮正徳, 佐藤守, 小寺義男, 野村文夫
  • Organizer: 第57回日本臨床検査医学会学術集会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2010-08-29
• [Presentation] 質量分析装置を用いた問題飲酒者診断法確立への基礎的検討 (2010)
  • Author(s): 曽川一幸, 野田健太, 小島良, 野村文夫
  • Organizer: 第59回日本医学検査学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-05-23
• [Book] アルコールと医学生物学 (2010)
  • Author(s): 曽川一幸, 他
  • Total Pages: 82-88
  • Publisher: 東洋書店
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/moldiag/clinical_proteomics_index.html
• [Patent(Industrial Property Rights)] コフィリンとその自己抗体との複合体の免疫測定方法、それに用いるキットおよびそれを用いた癌判定方法 (2010)
  • Inventor(s): 曽川一幸, 小島良, 野田健太, 宮崎勝, 吉富秀幸, 高野重紹, 野村文夫, 佐藤守
  • Industrial Property Rights Holder: 曽川一幸, 小島良, 野田健太, 宮崎勝, 吉富秀幸, 高野重紹, 野村文夫, 佐藤守
  • Industrial Property Number: 特許、2010-122204
  • Filing Date: 2010-05-28
• [Patent(Industrial Property Rights)] Novel Biomarkers of Biliary Tract Cancer (2010)
  • Inventor(s): 曽川一幸, 吉川真太郎, 横須賀收, 野村文夫
  • Industrial Property Rights Holder: 曽川一幸, 吉川真太郎, 横須賀收, 野村文夫
  • Industrial Property Number: 特許、2010-248021
  • Filing Date: 2010-11-05