Development of a single-session replacement therapy by the transplantation of personalized functional protein-producing intestinal organoids

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K19519
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 53:Organ-based internal medicine and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Okamoto Ryuichi 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (50451935)
• Project Period (FY): 2022-06-30 – 2024-03-31
• Keywords: 機能性神経内分泌腫瘍 / オルガノイド / 機能性分泌蛋白

Outline of Final Research Achievements

Through our series of study, it was confirmed that organoids derived from functional neuroendocrine tumors can be established from multiple different cases using established methods. Additionally, the cultivation progress and proliferation efficiency after establishment showed varying patterns depending on individual cases, suggesting a reflection of the gene expression patterns in the original tissues. Moreover, based on the gene expression patterns obtained from analyses using organoids derived from functional neuroendocrine tumors, candidate factors that regulate the expression of specific secreted proteins were identified. Comparative verification was also conducted regarding the appropriate models for transplanting these organoids, and conditions under which engraftment can be achieved with a certain efficiency were confirmed.

Free Research Field

消化器内科

Academic Significance and Societal Importance of the Research Achievements

特定の分泌蛋白について恒常的な発現制御機能を欠くとした機能性神経内分泌腫瘍について、同腫瘍由来のオルガノイドを樹立・解析するための手法を確立した。また複数症例の機能性神経内分泌腫瘍由来オルガノイドを比較検討することにより、機能性分泌蛋白に関する異なる制御機構を反映した遺伝子発現パターンに関する網羅的データの取得に成功し、これに基づき発現制御因子群を抽出する基盤を確立した。