Rapid and sensitive membrane-based immunoassay driven by multiple signal amplification mechanisms

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20476
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0401:Materials engineering, chemical engineering, and related fields
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Okuyama Hiroto 東京工業大学, 科学技術創成研究院, 助教 (80961101)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: バイオセンサー / 多孔質膜 / 抗原抗体反応

Outline of Final Research Achievements

In this study, biosensors with high sensitivity and practical rapidity were designed, using porous membranes as the substrate. This sensor enabled the detection of target biomolecules by both optical and pressure signals. The biosensor developed in this study was able to detect molecules with a sensitivity of pg/ml and ng/ml when optical and pressure signals were used as outputs, respectively.

Free Research Field

膜工学

Academic Significance and Societal Importance of the Research Achievements

本課題で開発したイムノセンサーは、圧力変化及び色変化をシグナルとして利用することができ、さらに両者の検出レンジは1000倍近く異なる。本センサーを応用することで、検出レンジの異なる複数の標的分子を、同時かつ異なるシグナルアウトプットで検出することが可能になると期待される。