High-throughput screening of orthogonal peptide pairs for rapid construction of high-performance fluorescent immunosensors

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20477
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0401:Materials engineering, chemical engineering, and related fields
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Yasuda Takanobu 東京工業大学, 科学技術創成研究院, 助教 (90963249)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: 抗体 / コイルドコイルペプチド / バイオセンサー

Outline of Final Research Achievements

A yeast and phage, carrying coiled-coil forming peptide were successfully fabricated, indicated that they enable to interact with each other and target population was enriched by using FACS. Some mutated peptide pairs suppressed the anti-parallel binding were designed, demonstrated that this mutant improves the performance of a probe.

Free Research Field

タンパク質工学

Academic Significance and Societal Importance of the Research Achievements

本研究で検討した酵母とファージを利用したペプチドのスクリーニング法は、より高効率に目的集団を濃縮できる条件について検討を重ねることで、新しい機能性ペプチドを獲得する手段として利用が期待される。また、今回新たに設計・合成した逆平行結合抑制ペプチドペアは、今後の高性能なQ-bodyの作製に資すると考えられる。