Affects of B cell deletion therapy on BBB

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20685
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0704:Neuroscience, brain sciences, and related fields
• Research Institution: Yamaguchi University
• Principal Investigator: Fujikawa Susumu 山口大学, 医学部附属病院, 助教 (40967113)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: 血液脳関門

Outline of Final Research Achievements

Currently, we are developing a BBB model with triple-cultured blood-brain barrier constituent cells on a novel membrane material (patent pending, details not disclosed). Prior to completing the BBB model using the new membrane material, we conducted a preliminary study using a monolayer BBB model with human brain microvascular endothelial cell lines to evaluate the effect of B cells on the transmigration of healthy human PBMCs. In this experimental setup, the removal of CD19-positive B cells did not affect the transmigration of healthy human PBMCs. We plan to evaluate the impact of B cells on PBMC transmigration using the BBB model with the new membrane material in future studies.

Free Research Field

神経免疫

Academic Significance and Societal Importance of the Research Achievements

中枢神経自己免疫疾患に対するCD19抗体やCD20抗体によるB細胞枯渇療法の作用機序は不明である。リンパ球同士の相互作用による自己反応性リンパ球の中枢神経浸潤制御という独自の視点から、中枢神経自己免疫疾患に対するCD19抗体やCD20抗体によるB細胞枯渇療法の作用機序を解明するための、独創的なin vitro BBBモデルを用いた研究手法を開発することができた。