Effects of cytokine-stimulated MSC-derived extracellular vesicles on bone-tendon healing of shoulder rotator cuff tears.

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20937
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0905:Surgery of the organs maintaining homeostasis and related fields
• Research Institution: Osaka University
• Principal Investigator: Hanai Hiroto 大阪大学, 医学部附属病院, 医員 (90964437)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: エクソソーム / 間葉系間質細胞 / 肩腱板断裂

Outline of Final Research Achievements

Mesenchymal stromal cells (MSCs) secrete small extracellular vesicles (sEVs), mainly exosomes, and are known to contribute to tissue repair by transmitting information between cells. MSCs have attracted attention as a regenerative medicine for musculoskeletal tissues such as rotator cuff tears. In this field, different sEVs secreted by MSCs under different culture conditions have not been evaluated. In this study, it was revealed that sEVs secreted by MSCs preconditioned with IFN-γ had altered secretion amount and protein composition compared to normal sEVs.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

近年，肩腱板断裂に対して，関節鏡を用いた修復術の発展により低侵襲治療が可能となってきたが，実際には20％程度の頻度で再断裂を合併し，術後の肩安静固定期間が数ヶ月に及んでいる現状がある．本研究で得られた研究成果は，今後臨床現場においても質の良い腱板の短期的な修復の新たな知見になると考えられる.