Development of an in vitro model of lymphatic vessels in photoaging for anti-aging product

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K21217
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0909:Sports sciences, physical education, health sciences, and related fields
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Okuyama Katsuki 東京薬科大学, 薬学部, 助教 (20967799)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: 光老化 / 細胞外マトリックス / リンパ管形成 / 皮膚科学

Outline of Final Research Achievements

UVB irradiation augmented degradation and carbonylation of type I collagen in an irradiation energy-dependent manner. We investigated the effect of UVB-irradiated type I collagen on the lymphatic actions of human dermal lymphatic vessel endothelial cells (HDLECs) in vitro. HDLECs were inhibited the cell migration and tube formation in UVB-irradiated collagen. In addition, β1-integrin production in HDLECs were decreased under these conditions. Therefore, the inhibition of cell migration and tube formation of HDLECs in the presence of UVB-irradiated collagen was attributed to a β1-integrin dependent manner.

Free Research Field

皮膚科学

Academic Significance and Societal Importance of the Research Achievements

本研究では、光老化したコラーゲンによるリンパ管形成機構の一端を明らかにした。コラーゲン光老化コラーゲンとリンパ管形成との関連性を明らかにしたことは、しわ・たるみを兆候とした光老化の分子機構解明に繋がるものと示唆された。さらに、本研究でのin vitro光老化リンパ管モデルを用いて新規素材のスクリーニングをすることは、リンパ管を標的としたアンチエイジング製品の新規開発に貢献するものと期待される。