Regulation of functions and differentiation of ES/iPS cells by designing cell-recognizable chimera matrices

2014 Fiscal Year Final Research Report

• Project/Area Number: 23220014
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Foundation for Advancement of International Science (2014); Tokyo Institute of Technology (2011-2013)
• Principal Investigator: AKAIKE Toshihiro 公益財団法人国際科学振興財団, 再生医工学バイオマテリアル研究所, 所長 (30101207)
• Co-Investigator(Kenkyū-buntansha): KOBATAKE Eiry 東京工業大学, 大学院総合理工学研究科, 教授 (00225484) ; NAGAOKA Masato 福井大学, テニュアトラック推進本部, 助教 (90397050) ; ISE Hirohiko 九州大学, 先導物質化学研究所, 准教授 (10324253) ; HARADA Ichiro 東京工業大学, 生命理工学研究科, 講師 (00361759)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: ES/iPS細胞 / 再生医工学 / 細胞用まな板 / 細胞マトリックス設計 / 神経細胞 / E-カドヘリン / N-カドヘリン / 均一系肝細胞反応

Outline of Final Research Achievements

Novel E-cadherin-based engineered extracellular matrix showed fascinating results: (1) highly homogeneous proliferation and differentiation of definitive endoderm cells under single-cell level; (2)striking effect of matrix-dependent cell sorting for isolation and enrichment of mature hepatocytes and neural cells for possible elimination of contaminated and poorly differentiated cells; (3) the unique opportunity for continuous monitoring of cellular behavior in different stages of differentiation.
Taken together, our novel recombinant ECM is advantageous for generating homogeneous population of differentiated cells without any enzymatic stress and cell sorting as well as proliferation. We established a novel biomedical field in cadherin biology named as “Cadherin-Matrix Engineering” which can be applied to “Cell-cooking Plate” for ES/iPS cells.

Free Research Field

複合領域