Advanced Micro Fluidic Engineering and Its Applications for High Sensitive Quantitative Measurements of Biomolecules

2015 Fiscal Year Final Research Report

• Project/Area Number: 23226010
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Measurement engineering
• Research Institution: Waseda University
• Principal Investigator: SHOJI Shuichi 早稲田大学, 理工学術院, 教授 (00171017)
• Co-Investigator(Kenkyū-buntansha): TAKEYAMA Haruko 早稲田大学, 理工学術院, 教授 (60262234) ; MIZUNO Jun 早稲田大学, ナノ・ライフ創新研究機構, 教授 (60386737) ; SEKIGUCHI Tetsushi 早稲田大学, ナノ・ライフ創新研究機構, 教授 (70424819)
• Co-Investigator(Renkei-kenkyūsha): HOSOKAWA Masato 早稲田大学, 理工学術院, 助教 (60722981) ; YOON Dong Hyun ナノ理工学研究機構, 助教 (70711498) ; SUZUKI Miho 埼玉大学, 理工学研究科, 准教授 (60222064) ; FUKUDA Takeshi 埼玉大学, 理工学研究科, 助教 (40509121) ; FUNATSU Takashi 東京大学, 大学院薬学系研究科, 教授 (00190124) ; TAKEDA Naoya 早稲田大学, 理工学術院, 准教授 (60338978) ; MORI Tetsushi 早稲田大学, 理工学術院, 助教 (00590100) ; EDAGAWA Yoshikuni 帝京平成大学, 薬学部, 教授 (50303607)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 計測システム

Outline of Final Research Achievements

In this study, the noble microfluidic device technology was developed in order to enable the optical high sensitive quantitative measurement of the micro luminescent sample as follows. 1) The micro droplet manufacturing technology of the various size. 2) The control technology of the micro flow. 3) The passive sorting technology of the micro droplet. Next, the measurement of the micro luminescent samples by systematizing microfluidic device was realized. 1) Insert a living cell to a droplet one by one, and analyze the gene of the environmental microbe. 2) Observation cellular growth and evaluate enzymatic reaction activity. Optical quantitative measurement was realized by this study.

Free Research Field

計測工学