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2013 Fiscal Year Final Research Report

Chemical Ecology of Mycorrhizal Matsutake

Research Project

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Project/Area Number 23380083
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionKyoto University

Principal Investigator

HIRAI Nobuhiro  京都大学, (連合)農学研究科(研究院), 教授 (00165151)

Co-Investigator(Kenkyū-buntansha) YAMADA Akiyoshi  信州大学, 農学部, 准教授 (10324237)
TANAKA Chihiro  京都大学, 大学院・農学研究科, 教授 (60263133)
Research Collaborator FUJITA Thoru  京都府農林水産技術センター
YOSHIKAWA Masami  京都府農林水産技術センター
Project Period (FY) 2011-04-01 – 2014-03-31
Keywords森林生態 / 保護 / 保全 / 菌根
Research Abstract

Matsutake is a typical ectomycorrhizal fungus associated with roots of red pine, and forms a fairy ring of fruiting bodies. The area of the fairy ring contains active mycorrhizas, and called as shiro. The previous study reported that that the shiro contained an antimicrobial substance. The antimicrobial substance has been remained unknown for 47 years. Our objective is isolation and identification of the antimicrobial substance from the shiro.
The shiro was extracted with 20% methanol, and the extract was purified with ODS and anion exchange resin to give an active fraction. The active fraction gave a crystalline, which was identified as oxalic acid by an X-ray analysis. However, the antimicrobial activity of the active fraction was clearly higher than that of oxalic acid. This means that the active fraction contains another substance which increases the activity of oxalic acid. The NMR and elemental analysis suggested that the substance was an inorganic compound.

  • Research Products

    (17 results)

All 2014 2013 2012 2011

All Journal Article (10 results) Presentation (6 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Synthesis of Japanese Boletus edulis ectomycorrhizae with Japanese red pine2014

    • Author(s)
      Endo, N., Kawamura, F., Kitahara, R., Sakuma, D., Fukuda, M., Yamada, A
    • Journal Title

      Mycoscience

      Volume: (in press)

  • [Journal Article] Transformation of the mushroom species Hypsizigus marmoreus, Flammulina velutipes, and Grifola rondosa by an Agrobacterium-mediated method using a universal transformation plasmid2013

    • Author(s)
      Hatoh, K., Izumitsu, K., Morita, A., Shimizu, K., Ohta, A., Kawai, M., Yamanaka, T., Neda, H., Ota, Y., Tanaka, C
    • Journal Title

      Mycoscience

      Volume: 54 Pages: 8-12

    • DOI

      10.1016/j.myc.2012.08.00215

  • [Journal Article] Trichoroma matsutake Y1 strain associated with Pinus densiflorae shows a gradient of in vitro ectomycorrhizal specificity with Pinaceae and oak hosts2013

    • Author(s)
      Yamada, A., Endo, N., Murata, H., Ohta, A., Fukuda, M
    • Journal Title

      Mycoscience

      Volume: 55 Pages: 27-34

    • DOI

      10.1016/j.myc.2-13.05.004

  • [Journal Article] Phylogenetic relationship and species delimitation of matsutake and allied species based on multilocus phylogeny and haplotype analyses2012

    • Author(s)
      Ota, Y., Yamanaka, T., Murata, H., Neda, H., Ohta, A., Kawai, M., Yamada, A., Konno, M., Tanaka, C
    • Journal Title

      Mycologia

      Volume: 104 Pages: 1369-1380

    • DOI

      10.3852/12-068

  • [Journal Article] A sheathing mycorrhiza between the tropical bolete Phlebopus spongiosus and Citrus maxima2012

    • Author(s)
      Phan, NDH., Yamada, A., Shimizu, K., Noda, K., Dang, LAT., Suzuki, A
    • Journal Title

      Mycoscience

      Volume: 52 Pages: 347-353

    • DOI

      10.1007/s10267-011-0177-5

  • [Journal Article] Ectomycorrhizal formation on regenerated somatic pine plants after inoculation with Tricholoma matsutake2012

    • Author(s)
      Yamanaka, T., Maruyama, T., Yamada, A., Miyazaki, Y., Kikuchi, T
    • Journal Title

      MushroomScience and Biotechnology

      Volume: 20 Pages: 93-97

  • [Journal Article] Dehydration tolerance in apple seedlings is affected by an inhibitor of ABA 8'-hydroxylase CYP707A. Dehydration tolerance in apple seedlings is affected by an inhibitor of ABA 8'-hydroxylase CYP707A2012

    • Author(s)
      Kondo, S., Sugaya, S., Sugawa, S., Ninomiya, M., Kittikorn, M., Okawa, K., Ohara H.,, Ueno, K., Todoroki, Y., Mizutani, M., Hirai, N
    • Journal Title

      J. Plant Physiol

      Volume: 169 Pages: 234-24

    • DOI

      10.1016/j.jplph.2011.09.007

  • [Journal Article] Synthesis and biological activity of amino acid conjugates of abscisic acid2011

    • Author(s)
      Todoroki, Y., Narita, K., Muramatsu, T., Shimomura, H., Ohnishi, T., Mizutani, M., Ueno, K., Hirai, N
    • Journal Title

      Bioorganic and Medicinal Chemistry

      Volume: 19 Pages: 1743-1750

    • DOI

      10.1016/j.bmc.2011.01.019

  • [Journal Article] Six-year monitoring of pine ectomycorrhizal biomass under a temperate monsoon climate indicates significant annual fluctuations in relation to climatic factors2011

    • Author(s)
      Okada, K., Okada, S., Yasue, K., Fukuda, M., Yamada, A
    • Journal Title

      Ecological Research

      Volume: 26 Pages: 411-419

    • DOI

      10.1007/s11284-011-0800-0

  • [Journal Article] Molecular organization of the mating type (Mat) locus of Exserohilummonoceras (Setosphaeria monoceras), a bioherbicide agent for Echinochloa weeds2011

    • Author(s)
      Morita, A, . Saitoh, Y., Izumitsu, K., Tanaka, C
    • Journal Title

      Mycoscience

      Volume: 53 Pages: 92-101

    • DOI

      10.1007/s10267-011-0141-4

  • [Presentation] アカマツ菌根圏土壌の抗菌活性物質2014

    • Author(s)
      西野勝俊, 城美沙緒, 大泉一也, 藤田徹, 山田明義, 田中千尋, 笹森貴裕, 時任宣博, 平井伸博
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学(生田キャンパス)
    • Year and Date
      2014-03-30
  • [Presentation] 灰色かび病菌のオートファジー関連遺伝子BcAtg1の機能解析2013

    • Author(s)
      住田卓也, 泉津弘佑, 田中千尋
    • Organizer
      第13回糸状菌分子生物学コンファレンス
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      20131120-21
  • [Presentation] マツタケ単一子実体由来の胞子分離株集団の選抜と培養特性2013

    • Author(s)
      早川記央, 村田仁, 山田明義
    • Organizer
      日本菌学会第57回大会
    • Place of Presentation
      東京農業大学
    • Year and Date
      20130607-09
  • [Presentation] Fruit-body formation of edible mycorrhizal Cantharellus from Japan in pot culture with pine host2013

    • Author(s)
      Ogawa, W., Endo, N., Yamada, A
    • Organizer
      7th International Workshop of Edible Ectomycorrhizal Mushroom
    • Year and Date
      2013-07-31
  • [Presentation] 植木鉢を用いたマツタケ菌根苗順化の試み2012

    • Author(s)
      小林 久泰, 山田明義
    • Organizer
      日本きのこ学会
    • Place of Presentation
      東京都, 東京農業大学
    • Year and Date
      2012-09-06
  • [Presentation] マツタケ研究における菌根合成法の展開2011

    • Author(s)
      山田明義
    • Organizer
      日本きのこ学会
    • Place of Presentation
      信州大学農学部
    • Year and Date
      2011-09-02
    • Invited
  • [Book] 菌類の事典 基礎編VI 生態15.3.3 菌類間の共生2013

    • Author(s)
      大園享司, 田中千尋
    • Publisher
      朝倉書店

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Published: 2015-06-25  

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