2013 Fiscal Year Final Research Report
Basic studies on development of a novel delivery system of materials into fish oocytes
| Project/Area Number |
23580243
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hokkaido University |
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Principal Investigator |
HIRAMATSU Naoshi 北海道大学, 大学院水産科学研究院, 准教授 (10443920)
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| Co-Investigator(Kenkyū-buntansha) |
GEN Koichiro 独立行政法人水産総合研究センター, 西海区水産研究所, 主任研究員 (80372051)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 魚類 / 受容体標的輸送 / ビテロジェニン / ビテロジェニン受容体 / 蛋白質相互作用 / 卵母細胞 |
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| Research Abstract |
A yolk precursor vitellogenin (Vg) is selectively incorporated from the maternal blood into oocyte following its binding to ovarian Vg receptor. This study demonstrated that Vg obtained from heterologous species, as well as Vg coupled with voluntary lipids, can be delivered to fish eggs and offsprings by injecting them into broodstocks. This indicated a potential use of fish Vg as a transporter of bioactive materials; the system can be universal to any fish species. This study also confirmed that such system was found to be applicable for the delivery of bioactive lipids and/or hydrophobic materials to eggs and offsprings across generations.
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[Journal Article] Ovarian expression and localization of a vitellogenin receptor with eight ligand binding repeats in the cutthroat trout (Oncorhynchus clarki)2013
Author(s)
Mizuta, H., Luo, W., Ito, Y., Mushirobira, Y., Todo T., Hara, A., Reading, B.J., Sullivan, C.V. and Hiramatsu, N.
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Journal Title
Comparative Biochemistry and Physiology, Part B
Volume: 166(1)
Pages: 81-90
DOI
Peer Reviewed
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[Presentation] Ovarian yolk formation in fishes : Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins2014
Author(s)
Hiramatsu, N., Todo, T., Sullivan, C.V., Reading, B.J., Matsubara, T., Ryu, Y.-W., Mizuta, H., Luo, W., Nishimiya, O., Mushirobira, Y. and Hara, A.
Organizer
10^<th> International Symposium on Reproductive Physiology of Fish (ISRPF)
Place of Presentation
Olhao, Portugal(基調講演として発表決定済)
Year and Date
20140525-30
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[Presentation] Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout2014
Author(s)
Mushirobira. Y., Mizuta, H., Luo, W., Todo, T., Hara, A., Reading, B.J., Sullivan, C.V. and Hiramatsu, N.
Organizer
10^<th> International Symposium on Reproductive Physiology of Fish (ISRPF)
Place of Presentation
Olhao, Portugal(ポスター発表として発表決定済)
Year and Date
20140525-30
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[Presentation] Yolk formation in fish : Multiple vitellogenins and their receptors2013
Author(s)
Hiramatsu N., Mizuta H., Luo W., Nishimiya O., Wu M., Mushirobira Y., Reading B.J., Sullivan C.V., Todo T. and Hara A.
Organizer
Diversification in Inland Finfish Aquaculture II (DIFAII)
Place of Presentation
University of South Bohemia, Vodnany, Czech Republic
Year and Date
20130924-26
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