Research and development toward the determination for the redox state of individual metal atoms in proteins

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657073
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: TAKEDA Kazuki 京都大学, 大学院・理学研究科, 講師 (30332290)
• Project Period (FY): 2011 – 2012
• Keywords: 異常分散 / 金属 / タンパク質

Research Abstract

Research and development toward the determination for the redox states of individual metal atoms in proteins from X-ray diffraction data sets collected near the absorption edges of the metals were performed by using the high-potential iron sulfur protein having the 4Fe-4S cluster and the Rieske protein having the 2Fe-2S cluster.

Research Products

• [Journal Article] Structure of a thermophilic cyanobacterial b_6f-type Rieske protein. (2012)
  • Author(s): Veit, S., Takeda, K., Tsunoyama, Y., Rexroth, D., Roegner, M. and Miki, K.
  • Journal Title: Acta Cryst Volume: D68 Pages: 1400-1408
  • DOI: DOI:10.1107/S0907444912034129
  • Peer Reviewed
• [Journal Article] 超高分解能・超高精度のタンパク質結晶構造解析 (2011)
  • Author(s): 竹田一旗、三木邦夫
  • Journal Title: 生物物理 Volume: 51 Pages: 96-97
  • DOI: DOI:10.2142/biophys.51.096
• [Journal Article] Dimer structure and conformational variability in the N-terminal region of an archaeal small heat shock protein, StHsp14.0. (2011)
  • Author(s): Takeda, K., Hayashi, T., Abe T., Hirano, Y., Hanazono, Y., Yohda, M. And Miki, K.
  • Journal Title: J. Struct. Biol Volume: 174 Pages: 92-99
  • DOI: DOI:10.1016/j.jsb.2010.12.006
  • Peer Reviewed
• [Remarks]
  • URL: http://www.kuchem.kyoto-u.ac.jp/organization/member/ktakeda.html