Fundamental investigation on the cell transplantation therapy to the chronic renal failure using the feline iPS cell

2013 Fiscal Year Final Research Report

• Project/Area Number: 23658266
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Clinical veterinary science
• Research Institution: Kitasato University
• Principal Investigator: HOSHI Fumio 北里大学, 獣医学部, 准教授 (00219164)
• Project Period (FY): 2011 – 2013
• Keywords: ネコ / 再生 / 腎臓

Research Abstract

Total-RNA was extracted from a feline tissue by using the AGPC method. Further total-RNA was purified to mRNA by the Oligo-dT latex beads method, and using this mRNA, the cloning of feline Klf4, Oct3/4, Sox2 and AQP2 gene was successful. The CDS of feline Klf4, Oct3/4 and Sox2 were inserted into pCl-neo Mammalian Vector. The fibroblast which it obtained from a feline skin tried the co-transfection of these vectors, but the iPS cell was not able to induce. While, feline AQP2 gene was inserted into pTrancer-EF-Bsd, and this vector was transfected into CrFK cell line and 293 cells line. It could isolate few high level expression GFP cell lines, and the increase of the water absorption was significantly higher than control CrFK cell line in the water absorption study using the cell.