2012 Fiscal Year Final Research Report
Regulation of intra- or extracellular glucocorticoid concentration by Lipocalin2 as a candidate marker for steroid dosage.
| Project/Area Number |
23790195
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Josai International University |
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Principal Investigator |
OZAWA Mika 城西国際大学, 薬学部, 助手 (40398558)
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| Project Period (FY) |
2011 – 2012
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| Keywords | プロテオミクス / バイオマーカー / ステロイド / タンパク質 / 相互作用 |
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| Research Abstract |
The aim of this study is to examine the hypothesis that lipocalin-2 binds to glucocorticoids, and regulates the concentration of intra- or extracellular free glucocorticoids. To examine the hypothesis, we established an assay for interaction between lipocalin -2 and glucocorticoids using surface plasmon resonance system. Our surface plasmon resonance analysis showed that lipocalin-2 interacts with dexamethasone dose-dependently , while the interaction of lipocalin-2 with prednisolone was not detected.. These results suggested that lipocalin-2 has a possibility of binding to specific glucocorticoids. We will examine the physiological role of lipocalin-2.
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