Identification of synovial sarcoma initiating cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791621
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA Taichi 北海道大学, 大学院・医学研究科, 助教 (90435959)
• Project Period (FY): 2011 – 2012
• Keywords: 滑膜肉腫 / 腫瘍幹細胞 / 新規治療標的分子

Research Abstract

We demonstrated that the cell surface antigen A is highly expressed in the sphere forming subpopulation of synovial sarcoma and correlate with the expression of stemness genes. Antigen A positive cells possessed high tumorigenic, self-renewing, and re-popularizing activity; therefore we concluded that the Antigen A can enrich synovial sarcoma initiating cells. By immunohistochemical analysis of 42 synovial sarcoma surgical specimens, Antigen A expression was well correlated with poor prognosis. Moreover, the specific inhibitor of antigen A could suppress the proliferation of synovial sarcoma cell-lines in vitro.
In this study, we first detect synovial sarcoma initiating cells and a specific marker, and these findings play a very important role about exploitation the new therapy against synovial sarcoma.

Research Products

• [Journal Article] Inhibition of GSH synthesis potentiates temozolomide-induced bystander effect in glioblastoma (2013)
  • Author(s): Kohsaka, S., Takahashi, K., Tanino, M., Kimura, T., Nishihara, H., Tanaka, S
  • Journal Title: Cancer Letter Volume: 331(1) Pages: 68-75
  • Peer Reviewed
• [Journal Article] Downregulation of miRNA-31 induces taxane resistance in ovarian cancer cells through increase of receptor tyrosinekinase MET (2013)
  • Author(s): Mitamura T., Watari H., Wang L., Kanno H., Hassan MK.,Miyazaki M.,Katoh Y., Kimura T., Tanino M., NishiharaH., Tanaka S., Sakuragi N
  • Journal Title: Oncogenesis Volume: 2 Pages: e40
  • Peer Reviewed
• [Journal Article] Expression of CD163 prevents apoptosis through the production of granulocyte colony-stimulating factor in meningioma (2013)
  • Author(s): Kanno H, Nishihara H, Wang L, Yuzawa S, Kobayashi H, Tsuda M, Kimura T, Tanino M, Terasaka S, Tanaka S
  • Journal Title: Neuro Oncol
  • Peer Reviewed
• [Journal Article] STAT3 inhibition overcomes temozolomide resistance in glioblastoma by downregulating MGMT expression (2012)
  • Author(s): Kohsaka, S., Wang, L., Yachi, K., Mahabir, R., Narita, T., Itoh, T., Tanino, M., Kimura, T., Nishihara H., Tanaka, S
  • Journal Title: Mol Cancer Ther Volume: 11 Pages: 1289-1299
  • Peer Reviewed
• [Journal Article] CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma (2012)
  • Author(s): Kimura, T., Wang, L., Tabu, K., Nishihara, H., Mashita, Y., Kikuchi, N., Tanino, M., Hiraga, H., Tanaka, S
  • Journal Title: Cancerinvest Volume: 30 Pages: 390-397
  • Peer Reviewed
• [Journal Article] Prognostic implication of histological oligodendroglial tumor component: clinicopathological analysis of 111 cases of malignant gliomas (2012)
  • Author(s): Kanno, H., Nishihara, H., Narita, T., Yamaguchi, S., Kobayashi, H., Tanino, M., Kimura, T., Terasaka S., Tanaka, S
  • Journal Title: PLoS One Volume: 7 Pages: e41669
  • Peer Reviewed
• [Journal Article] CRKL plays a pivotal role intumorigenesis of head and neck squamous cell carcinoma through the regulation of cell adhesion (2012)
  • Author(s): Yanagi, H.,Wang, L.,Nishihara, H., Kimura, T., Tanino, M.,Yanagi, T.,Fukuda, S.,Tanaka, S
  • Journal Title: Biochem Biophys Res Commun Volume: 418 Pages: 104-109
  • Peer Reviewed
• [Journal Article] Successful transplantation of rat hearts subjected to extended cold preservation with a novel preservation solution (2012)
  • Author(s): Wakayama, K.,Fukai, M.,Yamashita, K.,Kimura, T.,Hirokata, G.,Shibasaki, S.,Fukumori, D.,Haga, S. Sugawara, M.,Suzuki, T.,Taniguchi, M.,Shimamura, T.,Furukawa, H.,Ozaki, M.,Kamiyama, T.,Todo, S
  • Journal Title: Transpl Int Volume: 25 Pages: 696-706
  • Peer Reviewed
• [Presentation] from tissues to therapeutic reagents (2012)
  • Author(s): Shinya Tanaka, Shinji Kohsaka, Kouich Tabu, Lei Wang, Masumi Tsuda, Taichi Kimura, Mishie Tanino, Hiroshi Nishihara, Hiromichi Kimura: Molecular and pathological analysis of malignant glioma
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌ほか(札幌)
  • Year and Date: 20120919-21
• [Presentation] STAT3 inhibition overcomes temozolomide resistance in glioblastoma by downregulation of MGMT expression (2012)
  • Author(s): hinji Kohsaka, Lei Wang, Kazuhiro Yachi, Roshan Mahabir, Mishie Tanino, Taichi Kimura, Hiroshi Nishihara, Shinya Tanaka
  • Organizer: Cold Spring Harbor Laboratory Meeting; Mechanisms and Models of Cancer
  • Place of Presentation: Cold Spring HarborLaboratory (USA)
  • Year and Date: 20120814-18
• [Presentation] Identification and analysis of synovial sarcoma initiating cells. Cold SpringHarbor Laboratory Meeting (2012)
  • Author(s): Taichi Kimura, Lei Wang, Mishie Tanino, Hiroshi Nishihara, Shinya Tanaka
  • Organizer: Mechanisms and Models of Cancer
  • Place of Presentation: Cold Spring Harbor Laboratory (USA)
  • Year and Date: 20120814-18
• [Presentation] Thedistinct role of CRKI, CRKII and CRKL in tumorigenesis of human head and neck squamous cell carcinoma (2012)
  • Author(s): Hiroshi Nishihara, Hiroko Yanagi, Yusuke Fukushima, Teruki Yanagi, Lei Wang, Taichi Kimura, Mishie Tanino,Masumi Tsuda and Shinya Tanaka
  • Organizer: Mechanisms& Models of Cancer Meeting
  • Place of Presentation: Salk Institute(USA)
  • Year and Date: 20120801-04
• [Presentation] CD133 は AKT 経路を介して滑膜肉腫の造腫瘍能を負に制御する (2012)
  • Author(s): 木村太一 , 王磊, 平賀博明, 谷野美智枝, 西原広史, 田中伸哉
  • Organizer: 第92回北海道医学大会腫瘍系分科会(第106回北海道癌談話会)
  • Place of Presentation: 北大(札幌)
  • Year and Date: 2012-10-06