2013 Fiscal Year Final Research Report
Development of the articular cartilage injury therapy with the cell sheet
Project/Area Number |
23791666
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Orthopaedic surgery
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Research Institution | Tokai University |
Principal Investigator |
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Project Period (FY) |
2011 – 2013
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Keywords | 関節病学 / 再生医学 |
Research Abstract |
Background: We attempted regeneration of the articular cartilage using layered chondrocyte sheets prepared with temperature-responsive culture dishes.However, for clinical application, it is necessary to evaluate the characteristics of the cells in these sheets, identify their similarities to naive cartilage, and develop a technique to produce the cell sheets in shorter period. We paid attention to in vivo cartilaginous oxygen concentration. Methods: We made a cell sheet under various oxygen concentration environment and evaluated the characteristics. Result: We were able to manufacture the product stratification cartilage cells sheet which it was earlier and were full of extracellular matrices.
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Research Products
(40 results)
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[Journal Article] In vivo cell tracking by bioluminescence imaging after transplantation of bioengineered cell sheets to the knee joint2014
Author(s)
Yuko Takaku, Kunihiko Murai, Taku Ukai, Satoshi Ito, Mami Kokubo, Masaaki Satoh, Eiji Kobayashi, Masayuki Yamato, Teruo Okano, Mamoru Takeuchi, Joji Mochida, Masato Sato
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Journal Title
Biomaterials
Volume: 35(7)
Pages: 2199-2206
Peer Reviewed
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[Journal Article] Development of a novel vitrification method for chondrocyte sheets2013
Author(s)
Miki Maehara, Masato Sato, Masahito Watanabe, Hitomi Matsunari, Mami Kokubo, Takahiro Kanai,Michio Sato, Kazuaki Matsumura, Suong-Hyu Hyon, Munetaka Yokoyama, Joji Mochida and Hiroshi Nagashima
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Journal Title
BMC Biotechnol
Volume: 13(58)
Peer Reviewed
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[Journal Article] Studies of the humoral factors produced by layered chondrocyte sheets2012
Author(s)
Hamahashi K, Sato M, Yamato M, Kokubo M, Mitani G, Ito S, Nagai T, Ebihara G, Kutsuna T, Okano T, Mochida J
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Journal Title
J Tissue Eng Regen Med
Volume: (Published online in Wiley Online Library)
Peer Reviewed
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[Journal Article] Repair of articular cartilage defect with layered chondrocyte sheets and cultured synovial cells2012
Author(s)
Ito S, Sato M, Yamato M, Mitani G, Kutsuna T, Nagai T, Ukai T, Kobayashi M, Kokubo M, Okano T, Mochida J
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Journal Title
Biomaterials
Volume: 33(21)
Pages: 5278-5286
Peer Reviewed
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[Journal Article] Cartilage repair in transplanted scaffold-free chondrocyte sheets using a minipig model2012
Author(s)
Ebihara G, Sato M, Yamato M, Mitani G, Kutsuna T, Nagai T, Ito S, Ukai T, Kobayashi M, Kokubo M, Okano T, Mochida J
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Journal Title
Biomaterials
Volume: 33(15)
Pages: 3846-3851
Peer Reviewed
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