Control of membrane fouling based on structures of polysaccharides revealed by application of glyco-blotting

2014 Fiscal Year Final Research Report

• Project/Area Number: 24360212
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA Katsuki 北海道大学, 工学(系)研究科(研究院), 准教授 (10292054)
• Co-Investigator(Kenkyū-buntansha): HINOU Hiroshi 北海道大学, 先端生命科学研究院, 助教 (70333333) ; AIZAWA Tomoyasu 北海道大学, 先端生命科学研究院, 准教授 (40333596)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 膜ファウリング / ＭＢＲ / 多糖

Outline of Final Research Achievements

Enrichment of polysaccharides by glyco-blotting, in which polysaccharides are specifically purified via interactions between the aldehydes in the polysaccharides and aminooxy groups on glycoblotting beads, enabled MALDI-TOF/MS analysis at a high resolution. Structures of polysaccharides extracted from fouled membranes used in a pilot-scale MBR and those in the supernatant of the mixed liquor suspension in the MBR were investigated. It was demonstrated that the overlap between polysaccharides found in the supernatants and those extracted from the fouled membrane was limited, implying that polysaccharides that dominate in supernatants may not be important in membrane fouling in MBRs. Analysis using a bacterial carbohydrate database suggested that capsular polysaccharides (CPS) and/or lipo-polysaccharides (LPS) play an important role in the evolution of membrane fouling in MBRs.

Free Research Field

環境工学