Development of novel mosquito-control system using Bacillus thuringiensis Cry toxins

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Okayama University
• Principal Investigator: HAYAKAWA Tohru 岡山大学, 自然科学研究科, 助教 (30313555)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Bacillus thuringiensis / Mosquitocidal toxins / Cry toxin / Scorpion toxin / Synergism

Outline of Final Research Achievements

Mosquitos are vectors of important diseases like malaria and viral hemorrhagic fevers. Therefore, I tried to develop novel mosquito-control system using mosquitocidal toxin derived from soil bacterium, Bacillus thuringiensis. This system were supposed to be safe and are able to prevent, or at least delay the development of resistance-insects. In this study, I established the production systems for several mosquitocidal toxins (e.g. Cry11Ba, Cyt1Aa, AahIT, BjαIT) and analyzed their mosquitocidal mechanisms by several procedures (e.g. alanine scanning, binding assay). We also observed the synergism of mosquitocidal activity between Cry4Aa and Cry11Ba.

Free Research Field

昆虫病理学