2014 Fiscal Year Final Research Report
Production of gene-designed calves using combination of highly accurate gene analysis and advanced reproductive technologies
| Project/Area Number |
24380151
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUKUI Emiko 宇都宮大学, 農学部, 准教授 (20208341)
MATSUMOTO Hiromichi 宇都宮大学, 農学部, 准教授 (70241552)
NAGAO Yosikazu 宇都宮大学, 農学部, 教授 (70291953)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ウシ / 遺伝子多型 / 一塩基多型 / ゲノム / 体外受精 / 繁殖 / 生殖工学 / 経済形質 |
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| Outline of Final Research Achievements |
It was aimed to produce genetically designed calves efficiently using in vitro fertilization techniques of gametes from the parents which were accurately analyzed for whole genomes. The blood samples of 110 females from the herd of the Tochigi Prefectural Livestock & Dairy Experimental Center were highly accurately analyzed for single nucleotide polymorphism (SNP) of genotypes on economically valuable genes. The genotypes of same genes were also determined for frozen semen samples purchased of ten bulls. Based on the genotyping results, combinations of ova and sperm were planned for yields of genetically superior calves. Ova were obtained from ovaries of the females of the center using OPU technics or ovaries from the slaughterhouse in Utsunomiya-city. The resulting blastocysts, which should have the desired genotypes, were obtained using the previously reported IVM-IVF-IVC system. The produced blastocysts were transferred to recipient females, but no calf was obtained until now.
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| Free Research Field |
動物繁殖学
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