Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390331
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: The University of Tokushima
• Principal Investigator: KITAGAWA Tetsuya 徳島大学, ヘルスバイオサイエンス研究部, 教授 (80240886)
• Co-Investigator(Kenkyū-buntansha): KITAICHI Takashi 徳島大学, ヘルスバイオサイエンス研究部, 准教授 (20335813) ; NAKAGAWA Ryuji 徳島大学, 大学病院, 助教 (10403685) ; MAEDA Kazuhisa 独立行政法人国立病院機構四国こどもとおとなの医療センター(臨床研究部(成育)、臨, 総合周産期母子医療センター, 部長 (30294680) ; SUGANO Mikio 徳島大学, 大学病院, 医員 (70563807) ; NAKAYAMA Taisuke 徳島大学, ヘルスバイオサイエンス研究部, 特別研究員 (80582791) ; KUROBE Hirotsugu 徳島大学, ヘルスバイオサイエンス研究部, 助教 (30380083) ; KINOSHITA Hajime 徳島大学, 大学病院, 特任助教 (80611227)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 臍帯血 / 細胞移植 / 未熟肺血管床

Outline of Final Research Achievements

Objectives: To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods: Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice (BALB/c Slc-nu/nu) 4 weeks after MCT induction. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioimaging. Results: PH was significantly improved 4 weeks after hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions: The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH.

Free Research Field

心臓血管外科学