Quantitative anlysis on antigenic peptides from the tumor antigen and its application to a biomarker for cancer immunotherapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24501334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor immunology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: HOMMA SADAMU 東京慈恵会医科大学, 医学部, 教授 (50192323)
• Co-Investigator(Kenkyū-buntansha): IWAMOTO Takeo 東京慈恵会医科大学, 分子細胞生物学研究部, 准教授 (90568891) ; KOIDO Shigeo 東京慈恵会医科大学, 消化器・肝臓内科, 准教授 (70266617)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: がんワクチン / WT1 / 質量解析 / 膵癌 / LC/MS/MS / Mass imaging / 抗原性ペプチド

Outline of Final Research Achievements

Presentation of antigenic peptide of the tumor antigen on the HLA class I molecule is an essential factor for effective antigen-specific cancer vaccine. Using mass spectrometric analyses, identification and quantification of the HLA-A24 restricted antigenic peptide of Wilms' Tumor-1 (WT1) were tried in human pancreatic cancer zenograft tissue. Antigenic peptides binding to HLA class I molecules were obtained from the tumor cells by acid-elusion method and analyzed for detection of WT1 antigenic peptide using LC/MS/MS. WT1 antigenic peptide (CMTWNQMNL) was identified as a single ion peak and a standard curve for quantification could be generated. Furthermore, spots of WT1 antigenic peptide were observed in the tumor tissue by mass imaging analysis. Presentation of antigenic peptide of the tumor antigen on MHC class I molecules in tumor tissue could be defined by mass spectrometric examination.

Free Research Field

腫瘍免疫学