2013 Fiscal Year Final Research Report
Molecular mechanisms of anti-viral and anti-cancer activity of novel high mannose- binding lectins
| Project/Area Number |
24790101
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Yasuda Women's University |
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Principal Investigator |
SATO Yuichiro 安田女子大学, 薬学部, 講師 (60416427)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | レクチン / 抗ウイルス活性 / 抗がん活性 / オートファジー |
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| Research Abstract |
Bacterial lectin (PFL) was cloned, expressed in Escherichia coli and purified. Glycan array screening has revealed that PFL preferentially recognizes high mannose glycans with alpha1-3 Man that was highly exposed at the D2 position. PFL showed a potent anti-influenza virus activity by inhibiting the virus entry into cells via interaction with virus surface glycoprotein HA. Furthermore, PFL showed cytotoxicity against human gastric cancer MKN28 cells. Upon treatment with exogenous PFL, both integrin alpha2 and EGFR on the cell surface were internalized to the cytoplasm and accumulated to perinuclear region, together with the bound PFL. As the results of integrin/EGFR accumulation in cytoplasm, autophagic cell death was induced. This is a novel anti-cancer mechanism induced by the lectin.
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