Analysis of protein Cysteome modification in G protein-coupled receptor

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790252
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Kobe University
• Principal Investigator: ADACHI Naoko 神戸大学, バイオシグナル研究センター, 助教 (70604510)
• Co-Investigator(Renkei-kenkyūsha): SAITO Naoaki 神戸大学, バイオシグナル研究センター, 教授 (60178499)
• Research Collaborator: KAKU Mika 神戸大学, 大学院医学研究科, M2 ; UEDA Chie 神戸大学, バイオシグナル研究センター, 技術補佐員
• Project Period (FY): 2013-02-01 – 2015-03-31
• Keywords: パルミトイル化 / ベータ３アドレナリン受容体

Outline of Final Research Achievements

We have identified three palmitoylation sites of the human beta3-adrenergic receptor. Palmitoylation deficient mutants showed neither altered cAMP production nor receptor mislocalization. However, an irreversible palmitoylation inhibitor, 2-bromopalmitate greatly reduced receptor number on the plasma membrane. This result indicates that these palmitoylation are important for receptor stability. Since activation of beta3-adrenergic receptor facilitates lipid metabolism in fat cells therefore, protection of palmitoylated receptors might be a potential drug target against obesity.

Free Research Field

分子薬理学