Experimental Study on antitumor effect of AMPK activated regent on oral squamous cell carcinoma

2013 Fiscal Year Final Research Report

• Project/Area Number: 24792193
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kanazawa University
• Principal Investigator: KATO Koroku 金沢大学, 大学病院, 講師 (30444201)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 癌 / 細胞・組織 / AMPK / アポトーシス / 浸潤

Research Abstract

Apoptosis induction with AMPK activating agents was examined using an apoptosis detection kit, demonstrating marked apoptosis induction at 24 hours after administration.The mRNA expressions of cell growth factors were examined with AMPK activating agents, demonstrating the decreased expressions of mTOR and Akt1, Akt3 and the increased expressions of p53 and p21. Their protein expressions were also examined, demonstrating the decreased expression of phospho-mTOR over time and the increased expressions of p53 and p21.
The effects of AMPK activating agents on invasion were examined, demonstrating significant inhibitory effects on invasion in treated groups. The expressions of MMPs were examined, demonstrating the decreased expression of pro-MMP-2. Furthermore, the mRNA expression of ARK5 decreased over time.

Research Products

• [Journal Article] Loss of claudin-7 is a negative factor for invasion and metastasis in oral squamous cell carcinoma (2013)
  • Author(s): Yoshizawa K, Nozaki S, Kato A, Hirai M, Yanase M, Yoshimoto T, Kimura I, Sugiura S, Okamune A, Kitahara H, Noguchi N, Kato K, Ueki K, Kawashiri S
  • Journal Title: Oncology Reports Volume: 29 Pages: 445-450
  • Peer Reviewed
• [Journal Article] AMPK 活性化薬剤による口腔扁平上皮癌細胞の抗腫瘍効果メカニズムの解明 (2012)
  • Author(s): 加藤広禄, 北原寛子, 吉澤邦夫, 川尻秀一
  • Journal Title: 日本口腔組織培養学会誌 Volume: 22(1) Pages: 25-26
• [Presentation] 吉本泰輔, 北原寛子, 吉澤邦夫, 加藤広禄, 川尻 秀一 (2013)
  • Author(s): 吉本泰輔, 北原寛子, 吉澤邦夫, 加藤広禄, 川尻 秀一
  • Organizer: 日本口腔組織培養学会 設立50周年記念学術大会・総会
  • Place of Presentation: 日本歯科大学生命歯学部 九段ホール(東京)
  • Year and Date: 20131123-24
• [Presentation] 口腔扁平上皮癌の浸潤先進部のおけるCaveolin-1 発現に関する免疫組織化学的検討 (2013)
  • Author(s): 加藤広禄, 木村依世, 柳瀬瑞希, 杉浦史郎, 岡宗絢子, 北原寛子, 野口夏代, 吉澤邦夫, 中村博幸, 川尻秀一
  • Organizer: 第58回 日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡国際会議場 (福岡)
  • Year and Date: 20131011-13
• [Presentation] 口腔扁平上皮がん浸潤様式 4D 型におけるがん悪性化に関わる各遺伝子発現についての検討 (2013)
  • Author(s): 吉澤邦夫, 北原寛子, 野口夏代, 加藤広禄, 中村博幸, 川尻秀一
  • Organizer: 第37回 日本頭頸部癌学会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 20130613-14
• [Presentation] 口腔扁平上皮癌におけるVEGF-A 及 びVEGF-C 発現の免疫組織化学的検討 (2013)
  • Author(s): 柳瀬瑞希, 加藤広禄, 野口夏代, 吉澤邦夫, 北原寛子, 川尻秀一
  • Organizer: 第67回日本口腔科学会学術集会
  • Place of Presentation: 栃木県総合文化センター(栃木)
  • Year and Date: 20130522-24
• [Presentation] The AMPK activator AICAR inhibits the proliferation in oral squamous cell carcinoma (2013)
  • Author(s): Kato K, Noguchi N, Kitahara H, Kawashiri S
  • Organizer: Ninth AACR-Japanese Cancer Association Joint Conference : Breakthroughs in Basic and Translational Cancer Research
  • Place of Presentation: Hyatt Regency Maui (Maui), Hawaii
  • Year and Date: 20130221-25
• [Presentation] AMPK 活性化薬剤による口腔扁平上皮癌細胞の抗腫瘍効果の検討 (2012)
  • Author(s): 加藤広禄, 野口夏代, 吉澤邦夫, 北原寛子, 岡宗絢子, 杉浦史郎, 木村依世, 八木瑞希, 加藤阿希, 平井真理子, 川尻秀一
  • Organizer: 第57回 日本口腔外科学会総会・学術大会
  • Place of Presentation: パシフィコ横浜 (神奈川)
  • Year and Date: 20121019-21
• [Presentation] AMPK 活性化薬剤による口腔扁平上皮癌細胞の抗腫瘍メカニズムの検討 (2012)
  • Author(s): 加藤広禄, 吉澤邦夫, 北原寛子, 川尻秀一
  • Organizer: 第49回 日本口腔組織培養学会
  • Place of Presentation: 広島大学 (広島)
  • Year and Date: 20121019-21
• [Presentation] Claudin-7 expression as a prognostic factor correlates with mode of invasion in oral squamous cell carcinoma (2012)
  • Author(s): Yoshizawa K, Sugiura S, Okamune A, Kitahara H, Noguchi N, Kato K, Kawashiri S
  • Organizer: 17th World congress on advances in onclogy and 15th International symposium on molecular medicine
  • Place of Presentation: Creta Maris(Creta), Greece
  • Year and Date: 20121011-13