Pharmacological potential of curcumin analog targeting KSRP, a regulator of miRNA

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Akita University
• Principal Investigator: Shibata Hiroyuki 秋田大学, 医学(系)研究科(研究院), 教授 (50260071)
• Co-Investigator(Kenkyū-buntansha): IWABUCHI Yoshiharu 東北大学, 大学院薬学研究科, 教授 (20211766)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: クルクミン / mRNA / miRNA / KSRP

Outline of Final Research Achievements

Effects on mRNA and miRNA by curcumin analogs, GO-Y030 and GO-Y078, were examined. BCL-2, c-Myc and p53 were included in the affected transcripts by GO-Y030 among 41,058 transcripts in colorectal cancer cell line. Among 2,669 species of miRNAs in colorectal cancer cell line, 28 species and 11 species of miRNAs were up-regulated by GO-Y030 and GO-Y078, respectively. In endothelial cells, 470 species of mRNAs were up-regulated and 243 species were down-regulated. As for miRNA, has-miR-19b-3p was up-regulated in endothelial cells by GO-Y030, and 5 species of miRNAs were down-regulated by GO-Y078. KSRP contribution to the regulation of mRNA or miRNA expression was not elucidated at this present.

Free Research Field

臨床腫瘍学