2015 Fiscal Year Annual Research Report
二弁性大動脈弁における神経堤細胞の役割とその分子基盤の解析
| Project/Area Number |
25462147
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| Research Institution | Yamazaki Gakuen University |
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Principal Investigator |
富田 幸子 ヤマザキ学園大学, 公私立大学の部局等, 教授 (40231451)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 大動脈弁 / 神経堤細胞 / 二尖弁 / 1細胞解析 / Semapholin3E |
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| Outline of Annual Research Achievements |
心臓の半月弁、すなわち大動脈弁と肺動脈弁の形成は、心内皮由来間葉細胞、神経堤細胞由来細胞、二次心臓領域由来細胞が関与しているといわれている。弁周囲は心筋層と血管平滑筋層の両方が周囲環境としてある。最終年度では、ウズラ-ニワトリキメラを作成し、大動脈弁、肺動脈弁だけでなく大動脈弓の途中まで神経堤細胞が分布していることを見出した。神経堤マーカーを示す遺伝子改変マウス（Wnt1Cre-マウス）とR26REYFP-マウスとを交配し、流出路で蛍光発現する細胞1個ずつの性質を見極めたところcKitが神経堤細胞と関連していることを見出し、現在も検討中である。また流出路の冠血管入口部にSemapholin3E-PlexinD1受容体のシグナルが働いていることを見出し、発生途中の流出路大血管周囲に形成される脈管とリンパ管の異常がSemapholin3E ノックアウトマウスに見られた。本ノックアウトマウスでは心血管の異常も認めた。
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[Journal Article] Developmental genetic bases behind the independent origin of the tympanic membrane in mammals and diapsids.2015
Author(s)
Taro Kitazawa, Masaki Takechi, Tatsuya Hirasawa, Noritaka Adachi, Nicolas Narboux-Nême, Hideaki Kume, Kazuhiro Maeda, Tamami Hirai, Sachiko Miyagawa-Tomita, Yukiko Kurihara, Jiro Hitomi, Giovanni Levi, Shigeru Kuratani, and Hiroki Kurihara
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Journal Title
Nat Commun
Volume: 6
Pages: 6853-6858
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Distinct effects of Hoxa2 overexpression in cranial neural crest populations reveal that the mammalian hyomandibular-ceratohyal boundary maps within the styloid process.2015
Author(s)
Kitazawa T, Fujisawa K, Narboux-Nême N, Arima Y, Kawamura Y, Inoue T, Wada Y, Kohro T, Aburatani H, Kodama T, Kim KS, Sato T, Uchijima Y, Maeda K, Miyagawa-Tomita S, Minoux M, Rijli FM, Levi G, Kurihara Y, Kurihara H.
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Journal Title
Dev Biol
Volume: 402
Pages: 162 - 174
DOI
Peer Reviewed / Int'l Joint Research
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[Journal Article] Adult mice expressing a Braf Q241R mutation on an ICR/CD-1 background exhibit a cardio-facio-cutaneous syndrome phenotype.2015
Author(s)
Mitsuji Moriya1,8, Shin-ichi Inoue1,8, Sachiko Miyagawa-Tomita2,3, Yasumi Nakashima4, Daiju Oba1, Tetsuya Niihori1, Misato Hashi5, Hiroshi Ohnishi5, Shigeo Kure6, Yoichi Matsubara1,7 and Yoko Aoki.
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Journal Title
Hum Mol Genet
Volume: 24
Pages: 7349-7360
DOI
Peer Reviewed
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[Presentation] Semaphorin 3E-PlexD1 signaling regulate the proper coronary artery development.2016
Author(s)
Kazuaki Maruyama, Sachiko Miyagawa-Tomit , Yuichiro Arima, Daiki Seya, Kazuaki Naemura, Akiyoshi Uemura, Yutaka Yoshida, Mann Fanny, Yukiko Kurihara, Hiroki Kurihara
Organizer
Weinstein Cardiovascular Development and Regeneration Conference2016
Place of Presentation
Darham, USA
Year and Date
2016-05-19 – 2016-05-23
Int'l Joint Research
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[Presentation] Adult mice expressing a Braf Q241R mutation on an ICR/CD-1 background exhibit a cardio-facio-cutaneous syndrome phenotype.2016
Author(s)
Shin-ichi Inoue, Mitsuji Moriya, Sachiko Miyagawa-Tomita, Yasumi Nakashima, Daiju Oba, Tetsuya Niihori, Misato Hashi, Hiroshi Ohnishi, Shigeo Kure, Yoichi Matsubara and Yoko Aoki
Organizer
The 13th International Congress of Human Genetics
Place of Presentation
Kyoto International Conference Center, Kyoto
Year and Date
2016-04-03 – 2016-04-07
Int'l Joint Research
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[Presentation] Therapeutic research in a mouse model of cardio-facio-cutaneous syndrome.2016
Author(s)
Daiju Oba, Shin-ichi Inoue, Mitsuji Moriya, Yusuke Watanabe, Tetsuya Niihori, Sachiko Miyagawa-Tomita, Shigeo Kure, Toshihiko Ogura, Yoichi Matsubara, Yoko Aoki
Organizer
The 13th International Congress of Human Genetics
Place of Presentation
Kyoto International Conference Center, Kyoto
Year and Date
2016-04-03 – 2016-04-07
Int'l Joint Research
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[Presentation] がん原遺伝子BRAFの機能獲得性変異は先天性異常を引き起こす2015
Author(s)
井上晋一1, 守谷充司1,2, 宮川－富田幸子3, 中嶌八隅4, 大場大樹1, 新堀哲也1, 橋美里5, 大西浩史5, 呉繁夫2, 松原洋一1,6, 青木洋子1
Organizer
第38回日本分子生物学会年会
Place of Presentation
神戸ポートアイランド（兵庫県神戸市）
Year and Date
2015-12-01 – 2015-12-04
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