Molecular mechanism of growth retardation of mouse cells by shielding them from background radiation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25550035
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Osaka Prefecture University
• Principal Investigator: KAWANISHI Masanobu 大阪府立大学, 理学(系)研究科(研究院), 准教授 (70332963)
• Co-Investigator(Kenkyū-buntansha): ADACHI Jun 医薬基盤・健康・栄養研究所, プロテオームリサーチプロジェクト, 研究員 (20437255) ; SHIRAISHI Kazunori 大阪府立大学, 理学系研究科, 助教 (40347513) ; YAGI Takashi 大阪府立大学, 理学系研究科, 教授 (80182301)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 自然放射線 / 遮蔽 / 生物影響

Outline of Final Research Achievements

Does reduced level of background natural radiation alter the expression level of genes in mouse cells? To answer this question, using the DNA microarray we carried out comprehensive analyses of mRNA expression in mouse lymphoma L5178Y cells cultured in the shielding chamber surrounded by a 15 cm thick iron wall and a 10 cm thick paraffin wall that reduced the gamma ray and neutron levels in the chamber to 2% and 25% of the background levels, respectively. The altered expression levels of more than 300 genes were found, however none of them was not radioprotecion specific alteration.

Free Research Field

環境毒性学